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      • KCI등재

        Dynamic Protein Adsorption-Desorption Analysis of Contact Lenses in a Three-Dimensional-Printed Eye Model

        Wan-Hsin Chang,Pei-Yi Liu,Dai-En Lin,Yuan-Ting Jiang,Chien-Ju Lu,Yuan-Hao Howard Hsu 한국고분자학회 2022 Macromolecular Research Vol.30 No.1

        Adsorption of secreted protein on contact lenses is a dynamic and complex behavior. To understand this behavior, we used three-dimensional (3D) printing technology to create an eye model that simulated the anterior segment of the actual human eyeball. In this model, the fluid inlet was connected to a syringe pump to mimic the rate of human tear secretion and the outlet was connected to an ultraviolet- visible (UV-Vis) spectrophotometer. The experimental results revealed that the symmetrical eye model with a 180° inlet-outlet angle was suitable for dynamic analysis of protein adsorption. In this model, protein adsorption was slow and desorption was rapid. The contact lens was soaked in poly(2-methacryloyloxyethyl phosphorylcholine- co-butyl methacrylate) (PMB) to confirm the anti-protein adsorption property of this polymer through dynamic adsorption and desorption eye model analysis.

      • KCI등재

        Reduction of Physical Strength and Enhancement of Anti-Protein and Anti-Lipid Adsorption Abilities of Contact Lenses by Adding 2-Methacryloyloxyethyl Phosphorylcholine

        Wan-Hsin Chang,Pei-Yi Liu,Chien-Ju Lu,Dai-En Lin,Min-Hsuan Lin,Yuan-Ting Jiang,Yuan-Hao Howard Hsu 한국고분자학회 2020 Macromolecular Research Vol.28 No.12

        Biocompatible 2-methacryloyloxyethyl phosphorylcholine (MPC) can enhance the adsorption of water molecules and is therefore used for manufacturing contact lenses. This study investigated the mechanical strength, anti-protein deposition, and anti-lipid adsorption effects of MPC addition to contact lenses. Experimental contact lenses produced by copolymerizing multiple ratios of MPC to 2-hydroxyethyl methacrylate (HEMA) were analyzed. Atomic force microscopy revealed that MPC addition increased surface roughness. The anti-protein deposition and anti-lipid adsorption effects on poly(HEMA-MPC) polymers of various phosphorylcholine quantities were experimentally confirmed. The water content of the contact lenses was proportional to the MPC content in the polymer. The hydrated PC moiety of MPC drastically altered the network of the poly-HEMA polymer by inserting water molecules, which were trapped in the concave region of the surface. MPC addition had negative effects on all examined strength factors because of structural destabilization of the copolymer through water insertion. The anti-deposition effects of MPC were verified by examining the lysozyme and lipid adsorption abilities of the prepared contact lenses. Our results revealed that MPC enhanced interactions of the poly(HEMA-MPC) copolymer with water molecules; these interactions weakened the mechanical strength of the copolymer but markedly improved the anti-adsorption property of the biomolecules. The optimal proportion of HEMA–MPC for contact lenses is in the range 14.9%-28.5%.

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