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- 저자 : Chen Dongping (검색결과 3 건)
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Dongping Chen,Yashan Li,Yukang Wang,Jinlian Xu 한국유전학회 2021 Genes & Genomics Vol.43 No.2
Background Non-small cell lung cancer (NSCLC) is a major contributor of cancer-related mortality. Long non-coding RNAs (lncRNAs) are indicated to participate in the pathogenesis of NSCLC. Objective In this research, the efects of lncRNA HOXA transcript antisense RNA, myeloid-specifc 1 (HOTAIRM1) on NSCLC progression and underlying mechanism were revealed. Methods The expression levels of HOTAIRM1 and microRNA-498 (miR-498) were detected by quantitative real time polymerase chain reaction (qRT-PCR) in NSCLC tissues, cells or exosomes. The protein expression of CD63, CD81, hexokinase 2 (HK2) and ATP binding cassette subfamily E member 1 (ABCE1) was determined by western blot. Cell viability, apoptosis, migration and invasion were investigated by cell counting kit-8 (CCK-8), fow cytometry, transwell migration and invasion assays, respectively. Cell glycolysis metabolism was revealed by glucose uptake and lactate production assays and western blot analysis. The binding relationship between miR-498 and HOTAIRM1 or ABCE1 was predicted by DIANA-LncBase v2 and starBase online database, and identifed by dual-luciferase reporter assay. The efects of HOTAIRM1 on NSCLC growth in vivo were revealed by in vivo tumor formation assay. Results HOTAIRM1 expression was dramatically upregulated, whereas miR-498 expression was signifcantly downregulated in NSCLC tissues cells or exosomes as compared to control groups. Mechanistically, HOTAIRM1 knockdown repressed cell viability, migration, invasion and glycolysis metabolism, whereas induced cell apoptosis in NSCLC; however, miR498 inhibitor hindered these efects. Functionally, HOTAIRM1 functioned as a sponge of miR-498 and miR-498 targeted ABCE1. In addition, HOTAIRM1 silencing inhibited NSCLC growth in vivo by downregulating ABCE1 and upregulating miR-498 expression. Conclusions HOTAIRM1 knockdown repressed cell glycolysis metabolism and tumor development by reducing ABCE1 expression through sponging miR-498 in NSCLC, which provided a theoretical basis for further studying NSCLC progression.
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Abnormal Crowd Motion Detection with Hidden Conditional Random Fields Model
Dongping Zhang,Kaihang Xu,Yafei Lu,Chen Pan,Huailiang Peng 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.10
Crowd motion analysis in public places is an important research subject in the monitoring field. This paper proposes an approach for detecting abnormal crowd motion using Hidden Conditional Random Fields Model (HCRF). This approach derives variations of motion patterns from direction distribution of the crowd motion obtained by the optical flow and these variations are encoded with HCRF to allow for the detection of abnormal crowd motion. Modeling the temporal neighborhood relations in a video sequence based on HCRF can incorporate hidden states and label the video depending on long range observations. The experimental results show that this proposed algorithm can achieve better results than HMM and CRF.
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Xu Zhanyi,Yan Ru,Qian Jiali,Chen Dongping,Guo Yirong,Zhu Guonian,Wu Huiming,Chen Mengli 한국곤충학회 2022 Entomological Research Vol.52 No.11
RNA interference is an efficient approach for gene function identification and a potential novel strategy for selectively controlling pests. The red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae), is a major global storage pest, which causes great economic loss. Juvenile hormone esterase (JHE), a carboxylesterase, is responsible for the degradation of juvenile hormone. However, the knockdown effect of jhe on metamorphosis of pupae or adult in T. castaneum is unknown. In this study, we analyzed the expression profiles of Tc j h e in the larval stage, we found that Tc j h e was expressed throughout the whole larval instars and the expression levels were relatively high right after molting. Furthermore, we knocked down the expression of Tcjhe by injecting dsTc j h e , which significantly increased the mortality of adults and decreased the eclosion rate. In addition, abnormal developmental phenotypes, including wing deformitity, pupal–adult monsters and shrunken adults, were observed. Our finding indicates that JHE plays an important role in the metamorphosis and development in T. castaneum, suggesting that Tc j h e could be used as a potential target for the development of RNAi-based control strategies in T. castaneum.
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