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RISS 인기검색어
- 검색키워드
- 저자 : Chaeyoon HA (검색결과 2 건)
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Establishment of Potent Neutralizing Antibodies against SARS-CoV-2
Chaeyoon HA,Minjung CHOI,Se-ho PARK 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
SARS-CoV-2 is infected through the interaction between the spike protein of the virus and human angiotensin-converting enzyme 2 (hACE2), the receptor molecule of the host cell. Thus, most therapeutic antibodies have been developed to prevent the interaction between these two molecules. Despite the continuous development of therapeutic antibodies, there are only a few antibodies that effectively inhibit SARS-CoV-2 infection. In this study, we developed spike protein-specific antibodies to make effective SARS-CoV-2 therapeutic antibodies. We selected several hybridomas which are specific for Spike S1 protein. Then, potential clones were chosen by comparing S1 protein-specific affinity levels between selected hybridomas. To confirm whether these clones work as neutralizing antibodies, we tested the blocking effect by using SARS-CoV-2 pseudovirus to infect hACE2 expressing cells. As a result, total of 7 potent anti-S1 antibodies were obtained. Consequently, we expect these 7 clones to serve as therapeutic antibodies. To find whether it can inhibit infection in response to authentic SARS-CoV-2, in vitro and in vivo experiments are planned as further experiments.
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Development of Human-mouse Chimeric Antibodies Recognizing the SARS-CoV-2 Spike Protein
Minjung CHOI,Chaeyoon HA,Se-Ho PARK 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
The Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the identified cause of COVID-19, is regarded as an urgent problem throughout the world. Most neutralizing antibodies against SARS-CoV-2 focus on interfering with the contact between the spike (S) protein of the virus and its receptor, human angiotensin-converting enzyme 2 (hACE2). Here, we established a cell line expressing S protein of SARS-CoV-2 Wuhan type on its surface, as a substitute for an authentic virus, and immunized human IgG1 knock-in (hIgG1 KI) mice with previously mentioned cell line after irradiation to induce potent antibody responses. B cells were harvested from mice that received irradiated cell line and were measured by ELISA for serum titers of antigen-specific antibodies. The resulting positive and high antigen-specific antibody-producing B cells were further used in the hybridoma experiment. Positive clones from the hybridoma experiment were identified and thus, we suspect we have developed neutralizing antibody candidates that may be further studied and possibly be used as therapeutic agents against COVID-19.
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