http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Enhancement of Virus-induced Gene Silencing in Tomato by Low Temperature and Low Humidity
Da-Qi Fu,Ben-Zhong Zhu,Hong-Liang Zhu,Hong-Xing Zhang,Yuan-Hong Xie,Wei-Bo Jiang,Xiao-Dan Zhao,Yun-Bo Luo 한국분자세포생물학회 2006 Molecules and cells Vol.21 No.1
Virus-induced gene silencing (VIGS) is an attractive reverse-genetics tool for studying gene function in plants. We showed that silencing of a phytoene desaturase (PDS) gene is maintained throughout TRV-PDSinoculated tomato plants as well as in their flowers and fruit and is enhanced by low temperature (15°C) and low humidity (30%). RT-PCR analysis of the PDS gene revealed a dramatic reduction in the level of PDS mRNA in leaves, flowers and fruits. Silencing of PDS results in the accumulation of phytoene, the desaturase substrate. In addition, the content of chlorophyll a, chlorophyll b and total chlorophyll in the leaves of PDS-silenced plants was reduced by more than 90%. We also silenced the LeEIN2 gene by infecting seedlings, and this suppressed fruit ripenning. We conclude that this VIGS approach should facilitate large-scale functional analysis of genes involved in the development and ripening of tomato.
Static behavior of bolt connected steel-concrete composite beam without post-cast zone
Ying Xing,Yun Zhao,Qi Guo,Jin-feng Jiao,Qing-wei Chen,Ben-zhao Fu 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.38 No.4
Although traditional steel-concrete composite beams have excellent structural characteristics, it cannot meet the requirement of quick assembly and repair in the engineering. This paper presents a study on static behavior of bolt connected steel-concrete composite beam without post-cast zone. A three-dimensional finite element model was developed with its accuracy and reliability validated by available experimental results. The analysis results show that in the normal service stage, the bolt is basically in the state of unidirectional stress with the loss of pretightening can be ignored. Parametric studies are presented to quantify the effects of the post-cast zone, size and position of splicing gap on the behavior of the beam. Based on the studies, suggested size of gap and installation order were proposed. It is also confirmed that optimized concrete slab in mid-span can reduce the requirement of construction accuracy.