http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Bambang Sugiharto (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
-
Expression of somatic embryogenesis-related genes in sugarcane (Saccharum officinarum L.)
Ahdatu Uli Khikamil Maulidiya,Bambang Sugiharto,Parawita Dewanti,Tri Handoyo 한국작물학회 2020 Journal of crop science and biotechnology Vol.23 No.3
The low frequency of plantlet regenerates from the somatic embryogenesis (SE) callus in sugarcane becomes a problem to produce its seed. Plant growth regulators were able to increase the regeneration frequency of SE to normal plantlets, such as 2,4-dichlorophenoxyacetic acid (2,4-D) as promoting callus induction. Since molecular mechanisms involved SE in sugarcane have not been reported, expression of Baby Boom (BBM) and Leafy Cotyledon (LEC) genes related to SE had investigated. The effect of diff erence concentration of 2,4-D on callus induction and expression of somatic embryogenesis-related genes (BBM and LEC) in sugarcane were important information for the increasing quantity and quality of seed production. The percentage of callus formation and embryogenic callus in the Murashige and Skoog’s (MS) medium contained 4 mg/L 2,4-D after 6 weeks cultivation were 76% and 86%, respectively. The MS + 4 mg/L 2,4-D medium was recommended for the large-scale embryogenic callus production from sugarcane explant. The high-level expression of BBM and LEC was shown in the embryogenic callus, which suggested that the expressions of both genes were believed to play on the somatic embryogenesis regulation in sugarcane.
-
Dewi Puspa Arisandi,Fragaria Vesca Paradisa,Bambang Sugiharto,Sholeh Avivi,Wahyu Indra Duwi Fanata 한국작물학회 2020 Journal of crop science and biotechnology Vol.23 No.4
Breeding using the double haploid approach has been used in accelerating the rice breeding process. However, the main obstacle in the application of this technique in rice is the low level of callus formation and their regeneration into haploid plants. Based on these issues, this research is aimed at developing the best method for obtaining double haploid plants through anther culture using three kinds of aromatic rice of Indonesian local varieties. Anthers of Pendok, Genjah Arum, and Merah Wangi varieties were cultured in callus induction media which were either supplemented with different types of carbon sources, or auxins and ethylene inhibitors. However, the application of different sugar types did not significantly affect callus formation of Pendok and Genjah Arum. However, auxin treatment showed NAA to be more efficient for the callus induction of Pendok, while 2,4-D had no eff ect on callus induction in all tested varieties. The addition of ethylene inhibitor successfully induced callus formation of Merah Wangi varieties, while the rates of Pendok and Genjah Arum were not signifi cantly aff ected. Callus of three plant lines were then regenerated into plantlet in regeneration media. Of the three callus varieties used, only Pendok could regenerate into plantlets and grow into adult haploid plants.
-
Identification of Chinese Cabbage Sentrin as a Suppressor of Bax-Induced Cell Death in Yeast
( Sawitri Widhi Dyah ),( Slameto Slameto ),( Bambang Sugiharto ),( Kyung Min Kim ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.5
Studies into the cell death program termed apoptosis have resulted in new information regarding how cells control and execute their own demise, including insights into the mechanism by which death-preventing factors can inhibit Bax-induced caspase activation. We investigated high temperature stress-induced cell death in Brassica rapa. Using a yeast functional screening from a Brassica rapa cDNA library, the BH5-127 EST clone encoding an apoptotic suppressor peptide was identified. However, a phylogenic tree showed that BH5-127 clusters within a clade containing SUMO-1 (Small Ubiquitin-like Modifier- 1). BH5-127 was confirmed similar to have function to SUMO-1 as Fas suppression. Expression of BH5-127 showed that substantial suppression of cell death survived on SD-galactose-Leu--Ura- medium. The results suggest that BrSE (Brassica rapa Sentrin EST, BH5-127) is one of the important regulatory proteins in programming cell death, especially in the seedling stage of Chinese cabbage.
-
Fitria Ekawati Wulandari,Suherman,Tri Handoyo,김경민,Bambang Sugiharto 한국작물학회 2021 Journal of crop science and biotechnology Vol.24 No.1
The transgenic sugarcane overexpressing SoSPS1 gene increased sugar content which may alter the root exudation that interact with soil rhizosphere microorganism. The microbial plays an important role in biochemical cycling and nutrient transformation in the soil. Therefore, it is important to investigate the potential risk of the transgenic sugarcane on soil ecosystem. This study is designed to evaluate the impact of overexpressing gene for sucrose-phosphate synthesis ( So SPS1) on the bacterial diversity, enzyme activity and nutrient contents. The transgenic sugarcane showed no signifi cant difference in the population of culturable bacteria in the soil rhizosphere compared to non-transgenic counterpart. The populations of total, nitrogen-fixing and phosphate-solubilizing bacteria were similar in transgenic and non-transgenic sugarcane, although the bacterial population tends to increase at later sugarcane growth stage may due to the rise in nutrition from the root exudation. The PCR analysis showed no detection of horizontal gene fl ow in the bacteria. However, urease activity was significantly decreased in the transgenic sugarcane concomitant with lower nitrogen content in the soil rhizosphere at a later growth stage might due to the transgenic sugarcane lines increased nitrogen absorption for higher growth rate. Furthermore, the nutrient contents, such as organic carbon, phosphorus, potassium, magnesium and calcium, slightly varied in the soil rhizosphere but did not affected by the transgenic sugarcane. The results suggested that the transgenic sugarcane overexpressing SoSPS1 gene may not aff ect the bacterial diversity, nutrient contents and soil ecosystem.
ScienceON
KISS연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
