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Bipransh Kumar Tiwary,Anil Kumar,Ashis Kumar Nanda,Ranadhir Chakraborty 한국작물학회 2014 Journal of crop science and biotechnology Vol.17 No.3
The present study was conducted with the main objective to optimize petal yield from important marigold cultivars in WestBengal, and to standardize isolation of lutein from petals because these dietary xanthophylls are known to reduce the risks of agerelatedmacular degeneration (AMD) and cataracts. Six cultivars were studied viz. African marigold-Double (AFM-D), Africanmarigold-Single (AFM-S), African marigold-Orange (AFM-O), French marigold-Orange (FRM-O), French marigold-Double (FRMD),and LC (Local type), which withstand typical environmental conditions of northern West Bengal and produce flowers that do notvary in color and are relatively unaffected by pests and diseases. Lutein esters were extracted from milled marigold petals using nhexane. However, overall performance showed that LC is superior to the other cultivars. Pure lutein was obtained after saponificationwith 50% KOH. The re-crystallized lutein was characterized by UV-VIS, IR spectroscopy, and HPLC. A free-flowing lutein esterwas also formulated. This free-flowing lutein ester was found to be suitable to undergo commercialization or subsequent processing. An economic method for petal yield and isolation of lutein was thus standardized
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