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Female Breast Cancer Incidence and Mortality in Mexico, 2000-2010
Anaya-Ruiz, Maricruz,Vallejo-Ruiz, Veronica,Flores-Mendoza, Lilian,Perez-Santos, Martin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.3
The objective of this study was to investigate the recent incidence and mortality trends for breast cancer in Mexican females. Data between 2000 and 2010 from the Department of Epidemiology of the Ministry of Health, and International Agency for Research on Cancer (IARC) were analyzed. Age-standardized rates (ASRs) and annual percent changes (APCs) were calculated. The absolute incidence and mortality rates of breast cancer increased: 3,726 and 4,615 in 2000 to 8,545 and 4,966 in 2010, respectively. Incidence increased over time in all age groups tested, the 60-64 age group had the highest ASR (57.4 per 100,000 women in 2010), while the 20-44 age group had the lowest ASR (12.3 in 2010). The results show that incidence of breast cancer has increased in Mexico during last one decade, especially among older women, while the downturn observed in mortality mainly reflects improved survival as a result of earlier diagnosis and better cancer treatment.
Anaya-Ruiz, Maricruz,Bandala, Cindy,Perez-Santos, Jose Luis Martin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6
MicroRNAs (miRNAs) are small, non-coding RNAs (18-25 nucleotides) that post-transcriptionally modulate gene expression by negatively regulating the stability or translational efficiency of their target mRNAs. In this context, the present study aimed to evaluate the in vitro effects of miR-485 mimics in breast carcinoma T47D cells. Forty-eight hours after T47D cells were transfected with miR-485 mimics, an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was utilized to determine the effects on cell viability. Colony formation and cell migration assays were adopted to determine whether miR-485 affects the proliferation rates and cell migration of breast carcinoma T47D cells. Our results showed that ectopic expression of miR-485 resulted in a significant decrease in cell growth, cell colony formation, and cell migration. These findings suggest that miR-485 might play an important role in breast cancer by suppressing cell proliferation and migration.
miR-153 Silencing Induces Apoptosis in the MDA-MB-231 Breast Cancer Cell Line
Anaya-Ruiz, Maricruz,Cebada, Jorge,Delgado-Lopez, Guadalupe,Sanchez-Vazquez, Maria Luisa,Perez-Santos, Jose Luis Martin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5
MicroRNAs (miRNAs) are small, non-coding RNAs (18-25 nucleotides) that post-transcriptionally modulate gene expression by negatively regulating the stability or translational efficiency of their target mRNAs. In this context, the present study aimed to evaluate the in vitro effects of miR-153 inhibition in the breast carcinoma cell line MDA-MB-231. Forty-eight hours after MDA-MB-231 cells were transfected with the miR-153 inhibitor, an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was utilized to determine the effects of miR-153 on cell viability. Flow cytometry analysis and assessment of caspase 3/7 activity were adopted to determine whether miR-153 affects the proliferation rates and apoptosis levels of MDA-MB-231 cells. Our results showed that silencing of miR-153 significantly inhibited growth when compared to controls at 48 hours, reducing proliferation by 37.6%, and inducing apoptosis. Further studies are necessary to corroborate our findings and examine the potential use of this microRNA in future diagnostic and therapeutic interventions.
Cervical Cancer Trends in Mexico: Incidence, Mortality and Research Output
Anaya-Ruiz, Maricruz,Vincent, Ana Karen,Perez-Santos, Martin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.20
Purpose: To evaluate the recent incidence and mortality of and scientific research trends in cervical cancer in Mexican females. Materials and Methods: Data between 2000 and 2010 from the Department of Epidemiology of the Ministry of Health, and International Agency for Research on Cancer (IARC) of World Health Organization were analyzed, and age-standardized rates (ASRs) were calculated. In addition, scientific research data were retrieved from the Web of Science database from 2003 to 2012, using different terms related to cervical cancer. Results: The incidence rate decreased during last five years, while mortality rates showed an annual decrease of 4.93%. A total of 780 articles were retrieved, and the institutions with the majority of publications were National Autonomous University of Mexico (34.87%), Social Security Mexican Institute (16.02%), and National Institute of Cancerology (15%). The main types of research were treatment, diagnosis, and prevention. Conclusions: The above results show that incidence of cervical cancer decreased over time in Mexico during last five years; similarly, the downturn observed in mortality mainly reflects improved survival as a result of earlier diagnosis and cancer treatment. Also, this article demonstrates the usefulness of bibliometrics to address key evaluation questions and to establish priorities, define future areas of research, and develop cervical cancer control strategies in Mexico.
Innovation Status of Gene Therapy for Breast Cancer
Anaya-Ruiz, Maricruz,Perez-Santos, Martin Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9
Background: To analyze multi-source data including publications and patents, and try to draw the whole landscape of the research and development community in the field of gene therapy for breast cancer. Materials and Methods: Publications and patents were collected from the Web of science and databases of the five major patent offices of the world, respectively. Bibliometric methodologies and technology are used to investigate publications/patents, their contents and relationships. Results: A total of 2,043 items published and 947 patents from 1994 to 2013 including "gene therapy for breast cancer" were retrieved. The top five countries in global publication share were USA, China, Germany, Japan and England. On the other hand, USA, Australia, England, South Korea and Japan were the main producers of patents. The universities and enterprises of USA had the highest amount of publication and patents. Adenovirus- and retrovirus-based gene therapies and small interfering RNA (siRNA) interference therapies were the main topics both in publications and patents. Conclusions: The above results show that global research in the field of gene therapy for breast cancer is increasing and the main participants in this field are USA and Canada in North America, China, Japan and South Korea in Asia, and England, Germany, and Italy in Europe. Also, this article demonstrates the usefulness of bibliometrics to address key evaluation questions and define future areas of research.
Pokhrel, Anaya Raj,Chaudhary, Amit Kumar,Nguyen, Hue Thi,Dhakal, Dipesh,Le, Tuoi Thi,Shrestha, Anil,Liou, Kwangkyoung,Sohng, Jae Kyung Elsevier 2016 MICROBIOLOGICAL RESEARCH Vol.192 No.-
<P><B>Abstract</B></P> <P>The <I>dnrO</I> gene is the first regulator to be activated in the daunorubicin (DNR) biosynthesis pathway of <I>Streptomyces peucetius</I> ATCC 27952. DnrO is known for its self-repression capability while it activates rest of the DNR biosynthesis pathway through cascades of regulatory events. <I>S. peucetius</I> was found to contain no functional copy of <I>bldA</I>-tRNA while a detailed examination of <I>dnrO</I> codons reveals the presence of TTA codon, which is rarely encoded by <I>bldA</I>-tRNA. Therefore, for evaluating the role of <I>dnrO</I> in DNR production, multiple engineered strains of <I>S. peucetius</I> were generated by heterologously expressing <I>bldA, dnrO</I> and combination of <I>bldA</I> and <I>dnrO</I>. Using these strains, the effects of heterologously expressed <I>bldA</I> and overexpressed <I>dnrO</I> were evaluated on pathway specific regulators, mycelial densities and production of DNR. The results showed that the transcription level of <I>dnrO</I> and master regulator <I>dnrI</I>, was found to be elevated in <I>bldA</I> containing strain in comparison to <I>dnrO</I> overexpressed strain. The <I>bldA</I> containing strain produces 45.7% higher DNR than <I>bldA</I> deficient wild type strain from culture broth with OD<SUB>600</SUB> of 1.45 at 72h. Heterologous expression of <I>bldA</I>–tRNA is accounted for increased transcription levels of the DNR pathway specific regulators and enhanced DNR production.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Optimization of ultrasonic-assisted extraction of phenolic compounds from Justicia spicigera leaves
Luis Miguel Anaya-Esparza,Denı´ Ramos-Aguirre,Vı´ctor Manuel Zamora-Gasga,Elhadi Yahia,Efigenia Montalvo-Gonza´lez 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.4
A Box–Behnken design (Extraction-time, pulsecycle, sonication-amplitude) was employed to extract phenolic compounds from Justicia spicigera leaves by ultrasonic-assisted extraction. The muicle leaves extracts were analyzed measuring total phenolic compounds and antioxidant capacity. According to response surface methodology the optimal conditions of ultrasonic-assisted extraction to obtain the highest soluble phenolic content were 2 min (extraction time) for 0.7 s (pulse cycle) at 55% of sonication amplitude. Under these optimal conditions, the total phenolic content was higher when was used ultrasonic-assisted extraction (54.02 mg/g) than stirring (46.46 mg/g) and thermal decoction (47.76 mg/g); however, the antioxidant capacity from J. spicigera extracts did not increase by ultrasonic-assisted extraction. The extracts or aqueous infusions from J. spicigera leaves are used for therapeutic proposes, therefore the ultrasonic-assisted extraction is a useful technology to improve the extraction of phytochemicals from J. spicigera leaves.