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  • 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
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Ä Ö Ü ä ö ü ß
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      저자 : Alexander Krüttgen (검색결과 2 건)
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      • KCI등재

        Large inter-individual variability of cellular and humoral immunological responses to mRNA-1273 (Moderna) vaccination against SARS-CoV-2 in health care workers

        Alexander Krüttgen 대한백신학회 2022 Clinical and Experimental Vaccine Research Vol.11 No.1

        Purpose: Studies on the immune responses to severe acute respiratory syndrome coronavirus 2 vaccines are necessary to evaluate the ongoing vaccination programs by correlating serological response data and clinical effectiveness data. We performed a longitudinal immunological profiling of health care workers vaccinated with mRNA-1273 (Moderna, Cambridge, MA, USA). Half of these vaccinees had experienced a mild coronavirus disease 2019 (COVID-19) infection in the spring of 2020 (“COVID-recovered” cohort), whereas the other half of the vaccinees had no previous COVID-19 infection (“COVID-naive” cohort). Materials and Methods: Serum was drawn at multiple time points and subjected to assays measuring anti-Spike immunoglobulin G (IgG), avidity of anti-Spike IgG, avidity of anti-receptor binding domain (RBD) IgG, virus neutralizing activity, and interferon-ɣ release from stimulated lymphocytes. Results: Between both cohorts and within each cohort, we found remarkable inter-individual differences regarding cellular and humoral immune responses to the Moderna mRNA-1273 vaccine. Conclusion: First, our study indicates that the success of mRNA-1273 vaccinations should be verified by serological assays in order to identify “low-responders” to vaccination. Second, the kinetics of anti-S IgG and neutralizing activity correlate well with clinical effectiveness data, thus explaining incipient protection against infection 2 weeks after the first dose of mRNA-1273 in COVID-naive vaccinees. Third, our IgG-avidity data indicate that this incipient protection is mediated by low-avidity anti-RBD IgG and low-avidity anti-S IgG.

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        Severity of adverse reactions is associated with T-cell response in mRNA-1273 vaccinated health care workers

        Hanna Klingel,Maike Lauen,Alexander Krüttgen,Matthias Imöhl,Michael Kleines 대한백신학회 2022 Clinical and Experimental Vaccine Research Vol.11 No.1

        Knowledge about mRNA-1273 elicited T-cell response is limited. We investigated adverse reactions and interferon gamma release by specific T-cells among mRNA-1273 vaccinated health care workers. Seven to 13 weeks after complete vaccination low levels of specific Tcells were detected not correlating with antibody response. Severity of symptoms after first and number of symptoms after second immunization were associated with T-cell response. Assessment of T-cell response in addition to antibody response is crucial because even few specific T-cells could add to protection against infection. Investigation of mRNA-1273 induced inflammatory processes might help improve reactogenicity and immunogenicity

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