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RISS 인기검색어
- 검색키워드
- 저자 : Abdul Mujeeb Kazi (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
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Rasheed, Awais,Mahmood, Tariq,Kazi, Alvina Gul,Ghafoor, Abdul,Mujeeb-Kazi, Abdul 한국작물학회 2012 Journal of crop science and biotechnology Vol.15 No.1
The objective of this study was to identify allelic variations at $Glu-1$ loci of wheat ($Triticum$ $aestivum$ L.) advanced lines derived from hybridization of bread wheat and synthetic hexaploid wheats (2n = 6x = 42; AABBDD). Locally adapted wheat genotypes were crossed with synthetic hexaploid wheats. From the 134 different cross combinations made, 202 $F_8$ advanced lines were selected and their HMW-GS composition was studied using SDS-PAGE. In total, 24 allelic variants and 68 HMW-GS combinations were observed at $Glu-A1$, $Glu-B1$, and $Glu-D1$ loci. In bread wheat, the $Glu-D1$ locus is usually characterized by subunits 1Dx2+1Dy12 and 1Dx5+1Dy10 with the latter having a stronger effect on bread-making quality. The subunit 1Dx5+1Dy10 was predominantly observed in these advanced lines. The inferior subunit 1Dx2+1Dy12, predominant in adapted wheat germplasm showed a comparative low frequency in the derived advanced breeding lines. Its successful replacement is due to the other better allelic variants at the $Glu-D1$ locus inherited in these synthetic hexaploid wheats from $Aegilops$ $tauschii$ (2n = 2x = 14; DD).
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The Low Down on Association Mapping in Hexaploid Wheat (Triticum aestivum L.)
Sajjad, Muhammad,Khan, Sultan Habibullah,Kazi, Abdul Mujeeb 한국작물학회 2012 Journal of crop science and biotechnology Vol.15 No.3
The past few decades have witnessed hundreds of family-based linkage studies mapping for numerous traits but only a limited number of QTLs were actually cloned, tagged, or used for marker-assisted selection. Although providing valuable information, this conventional approach cannot be scaled up to underpin the incredible amount of phenotypic variation in the form of 266, 589 hexaploid wheat accessions maintained in public germplasm collections. Association mapping has recently emerged as an alternative and more powerful mapping approach where a natural population is surveyed to determine marker-trait associations using linkage disequilibrium (LD). After its first application for milling quality in 2006, association mapping studies in hexaploid wheat are being extended to tag yield traits, protein quality, and tolerance to biotic and abiotic stresses. Advances in genotyping technology and statistical approaches greatly accelerated the shift from conventional linkage-based mapping to LD-based association mapping. Association mapping stands out because of simultaneous utilization of a large number of ex situ-conserved natural variation due to historical recombination events accumulated over centuries.
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Awais Rasheed,Tariq Mahmood,Alvina Gul Kazi,Abdul Ghafoor,Abdul Mujeeb Kazi 한국작물학회 2012 Journal of crop science and biotechnology Vol.15 No.1
The objective of this study was to identify allelic variations at Glu-1 loci of wheat (Triticum aestivum L.) advanced lines derived from hybridization of bread wheat and synthetic hexaploid wheats (2n = 6x = 42; AABBDD). Locally adapted wheat genotypes were crossed with synthetic hexaploid wheats. From the 134 different cross combinations made, 202 F 8 advanced lines were selected and their HMW-GS composition was studied using SDS-PAGE. In total, 24 allelic variants and 68 HMW-GS combinations were observed at Glu-A1, Glu-B1, and Glu-D1 loci. In bread wheat, the Glu-D1 locus is usually characterized by subunits 1Dx2+1Dy12and 1Dx5+1Dy10 with the latter having a stronger effect on bread-making quality. The subunit 1Dx5+1Dy10 was predominantly observed in these advanced lines. The inferior subunit 1Dx2+1Dy12, predominant in adapted wheat germplasm showed a compara-tive low frequency in the derived advanced breeding lines. Its successful replacement is due to the other better allelic variants at the Glu-D1 locus inherited in these synthetic hexaploid wheats from Aegilops tauschii (2n = 2x = 14; DD).
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The Low Down on Association Mapping in Hexaploid Wheat (Triticum aestivum L.)
Muhammad Sajjad,Sultan Habibullah Khan,Abdul Mujeeb Kazi 한국작물학회 2012 Journal of crop science and biotechnology Vol.15 No.3
The past few decades have witnessed hundreds of family-based linkage studies mapping for numerous traits but only a limited number of QTLs were actually cloned, tagged, or used for marker-assisted selection. Although providing valuable information, this conventional approach cannot be scaled up to underpin the incredible amount of phenotypic variation in the form of 266, 589 hexaploid wheat accessions maintained in public germplasm collections. Association mapping has recently emerged as an alternative and more powerful mapping approach where a natural population is surveyed to determine marker-trait associations using linkage disequilibrium (LD). After its first application for milling quality in 2006, association mapping studies in hexaploid wheat are being extended to tag yield traits, protein quality, and tolerance to biotic and abiotic stresses. Advances in genotyping technology and statistical approaches greatly accelerated the shift from conventional linkage-based mapping to LD-based association mapping. Association mapping stands out because of simultaneous utilization of a large number of ex situ-conserved natural variation due to historical recombination events accumulated over centuries.
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