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Tissue Microarray를 이용한 원발 신장암 조직과 전이 조직 간의 조직생물학적 표지자(Tissue-BasedBiomarker)발현의 상관성에 관한 연구
김성한(Sung Han Kim),박원서(Weon Seo Park),박은영(Eun Young Park),박보람(Boram Park),주정남(Jungnam Joo),정재영(Jae Young Joung),서호경(Ho Kyung Seo),이강현(Kang Hyun Lee),정진수(Jinsoo Chung) 대한비뇨기종양학회 2016 대한비뇨기종양학회지 Vol.14 No.3
Purpose: The study was aimed to determine the correlations of tissue-based biomarker expressions between primary and metastatic specimens of renal cell carcinoma and with several well-known prognostic clinicopathological parameters. Materials and Methods: The immunohistochemistry (IHC) was used to determine the expression levels of 9 tissue-based markers calculated in H-score expressed by percentage of expression multiplied by the intensity score (0, 1, 2, and 3 points). Using 17 patients’ 38 specimens paired with primary renal lesion and its metastatic lesions collected between 2004 and 2015, Tissue microarray with IHC was performed with BAP1, PBRM1, pS6, PTEN, TGase2, PD-L1, CA9, PSMA, and Ki-67 on formalin-fixed paraffin-embedded sections. Pearson correlation and accuracy test were performed to analyze the correlation between primary and metastatic tissues. Results: The 17 patients’ mean age was 56.9 years old, mean tumor size was 7.9 cm, and the male to female ratio was 13:4 (76.5%:23.5%), respectively. Three patients had 2, 3, and 3 metastatic tissues, and the rest of 14 patients had only one metastatic tissue. The H-score (PSMA and Ki67) and intensity score (pS6 and PSMA) showed that some differential significant markers were identified which had statistical correlations of expression levels between primary and metastatic lesions among 9 markers. However, no real correlation of PSMA, Ki67, and pS6 markers were found their expressions of between primary and metastatic tissues because of their skewed expressions. Conclusions: Tissue markers failed to correlate their expression levels in primary lesions with those of metastatic lesions.