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조상래(Sang Nae Cho) 한국예방수의학회 1998 예방수의학회지 Vol.22 No.2
MPB70항원은 우형결핵균(Mycobacterium bovis)의 주요 분비항원으로 우형결핵균에 특이하여 우결핵의 혈청진단에 응용성이 비교적 높은 것 중의 하나이다. 본 연구에서는 MPB70 단백질의 혈청진단 유용성 여부를 평가하기 위하여 유전자재조합 기법으로 Escherichia coli에서 MPB70 단백질을 생산하여 그 항원성을 조사하였다. 유전자은행에 등록된 MPB70유전자의 핵산염기서열을 근거로 primer를 준비하였으며, polymerase chain reaction cloning법으로 pQE-30 expression vector에 MPB70 유전자를 클론하여 E. coli에서 발현시켰다. 유전자재조합 (recombinant: r) MPB70 단백질은 25 kDa으로 Western blotting 분석에서 우결핵 양성반응우의 혈청과 반응하였으나 우결핵 음성우의 혈청과는 반응하지 않아 rMPB70 단백질의 항원성과 특이성을 확인하였다. 효소결합면역분석법(ELISA)을 이용하여 rMPB70 단백질에 대한 항체검사를 시행한 결과 우결핵 양성반응우 29두 가운데 26두 (89.7%)에서 양성기준 흡광도를 초과하였으며, 우결핵 음성우 30두 모두 음성반응을 나타냈다. 따라서 본 연구에서 생산하여 정제한 rMPB70 단백질항원을 이용한 ELISA검사법은 비교적 높은 민감도와 특이도를 나타내 추후 우결핵 진단의 보조검사로 이용될 수 있음을 제시하였다.
Keynote Lectures : 국내 주요 인수공통전염병의 현황 : KL-2 ; Mycobacterial Infections in Humans as Zoonoses
조상래 ( Sang Nae Cho ) 대한인수공통전염병학회 2014 창립총회 및 학술대회 초록집 Vol.2014 No.1
Tuberculosis (TB), caused by Mycobacterium tuberculosis, has been one of three major infectious diseases in the world inflicting more than 8.7 million new cases and 1.3 million deaths each year. M. tuberculosis was reported to transmit from humans to a variety of animals including dogs, cattle, non-human primates, elephants, and birds, and secondary transmission of M. tuberculosis between animals was also reported resulting in back transmission to humans as a zoonosis. Meanwhile, bovine TB, caused by M. bovis, has been on the top list of zoonoses comprising about 30% of human tuberculosis in Europe before introduction of pasteurization of milk in the early 20th century. Although WHO claims that only 3.1% of all human TB cases are due to M. bovis infection, its rate varies depending of countries from 0.5 ~ 2.0% in the US and European countries to 5% in Nigeria, 7% in Uganda, and 13.8% in Mexico. M. bovis also can infect much wider range of animals, particularly wildlife animals as natural hosts and reservoirs, notably white-tailed deer, African buffalo, brush tail possum, European boar, Eurasian badger. Therefore, these animals can be also a source of human infection of M. bovis. Although there is still a controversy, M. avium subspecies paratuberculosis has been claimed as a cause of a portion of Crohn’s disease in humans. In addition, there are as high as 40 mycobacterial species including M. avium, M. kansasii, which can be found in animal tissues such as lymph nodes of cattle, which are potential source of human infections. Although chances are very rare in Korea, active control programs against mycobacterial infections, particularly M. bovis infection in animals are desirable to prevent further human infection considering that mycobacteria can survive in biofilm, soil, and amoeba in water.