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Immunocytochemical detection of HPV16 E7 in cervical smear
전주홍,조성엽,Kye-Yong Song,Noh-Hyun Park,Heun-Soo Kang,Yung Dai Kim,김인규,신동명 생화학분자생물학회 2007 Experimental and molecular medicine Vol.39 No.5
Cervical cancer is characterized by a long period of preclinical dysplasia or carcinoma in situ progressing into invasive cancer. Although Papanicolaou (Pap) smear test has contributed significantly to the early detection of precursor lesions, the cytological screening has inherent problems that produce considerable false negative/ positive results. Since the infection of high-risk type of human papillomavirus (HPV) is strongly associated with cervical cancer, we investigated the feasibility of an immunostaining test to detect cells infected by HPV in cervical smear. We produced monoclonal antibodies against HPV16 E7 in mice by repeated injections with the recombinant HPV16 E7. Western blot analysis and immunocytochemical assay demonstrated that the selected monoclonal antibody, mAb (130-9-7), reacts specifically with cultured cervical cancer cell lines infected by HPV16. Specific staining was observable with the HPV16-positive smear specimens obtained from the cervical cancer patients, whereas no staining was detected with the HPV-negative smear specimens. To achieve the desired sensitivity, specificity and reproducibility, we modified and optimized the conventional immunocytochemical procedure for cervical smear specimens. Our results suggest that this immunostaining method for detecting high-risk HPV in cervical smear may be used as a strategy to distinguish a high-risk group, especially those patients with low grade cytological abnormality.
전주홍,이혜진,장기용,김채완,신동명,조성엽,여의주,박상철,김인규 생화학분자생물학회 2004 Experimental and molecular medicine Vol.36 No.6
The treatment of cystamine, a transglutaminase (TGase) inhibitor, has beneficial effects in several diseases including CAG-expansion disorders and cataract. We compared the inhibition characteristics of cystamine with those of cysteam ine, a reduced form of cystamine expected to be present inside cells. Cystamine is a more potent inhibitor for TGase than cysteamine with different kinetics pattern in a nonreducing condition. By contrast, under reducing conditions, the inhibitory effect of cystamine was comparable with that of cysteamine. However, cystamine inhibited intracellular TGase activity more strongly than cysteamine despite of cytoplasmic reducing environment, suggesting that cystamine itself inhibits in situ TGase activity by forming mixed disulfides.
Improved immunodetection of human papillomavirus E7
전주홍,조성엽,김채완,신동명,권준철,최경호,김인규 생화학분자생물학회 2002 Experimental and molecular medicine Vol.34 No.6
Human papillomavirus E7 (HPV E7) is a viral on-coprotein that plays an important role in cervical carcinogenesis through binding with retinoblas-toma protein (Rb). Inactivation of Rb by E7 is necessary but not sufficient for cellular trans-formation, suggesting other protein-protein inter-actions are required for E7-mediated celular trans-formation aside from the interaction with Rb. HPV E7 have been limited by its poor imuno-reactivity. In this report, we show that the fixation of purified recombinant HPV E7 on blotted nitro-celulose membrane with glutaldehyde markedly enhanced the imunoreactivity of HPV E7 protein. Using HeLa and Caski cel line which are infected with HPV 18 and HPV 16, respectively, we de-monstrated that native HPV E7 proteins also could be detected by this method. These results there-fore can provide the experimental conditions for detection of HPV E7 proteins with greater sensi-
전주홍,박기광,조경엽,이상대,송정록 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.1
Bony defects of mandible caused by trauma, infection, or tumor resection have been reconstructed by autologous bone graftings. These reconstruction can restore facial contour and integrity of the oral cavity. However, it is often necessary to reduce the bulk of bone to allow soft tissue coverage of the graft. Therefore, the construction of removable dentures in this situation is fraught with frustation. The use of osseointegrated implants is a method to allow improved anchorage and support for the successful fabrication of dental protheses. We report two cases of the use of osseointegrated implants of the Branemark type(3i), in conjunction with bone grafting to the lower jaw.
대한구강악안면외과학회지를 위한 데이터베이스 관리 시스템
전주홍 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2
Dental research and writing for publication are based on a collection and arrangement of related literatures. The author developed DBMS(Database Management System) for articles of the Journal of Korean Academy of Oral and Maxillofacial Surgery. The computer program was constructed by dBASE Ⅲ^+ and Clipper using personal computer. This DBMS consisted of two parts. One is database including the information for individual articles, the other is the programs to store and retrieve references easily and rapidly. I expect that many oral and maxillofacial surgeons may utilize this DBMS to get the information of articles published in the Journal of Korean Academy of Oral and Maxillofacial Surgery.
박기광,조경엽,전주홍 大韓顎顔面成形再建外科學會 1995 Maxillofacial Plastic Reconstructive Surgery Vol.17 No.2
Chylous fistula is a rare complication occurring after radical neck dissection. Previous reports on neck dissection described an incidence of about 1% to 2%. We report a case of chylous fistula that occurred after radical neck dissection for squamous cell carcinoma of left lower gingiva and mandible in a 52-year-old man. We successfully managed the fistula by the following conservative measures : bed rest with head elevation, continuation of closed suction drainage, and dietary management to decrease the rate of chyle formation.
전주홍(Ju Hong Jeon),정창우(Chang Woo Jung),문재필(Jae Pil Moon) 대한구강악안면외과학회 1997 대한구강악안면외과학회지 Vol.23 No.1
A retrospective study was made of 10 consecutive patients who underwent mandibular reconstruction with PCBM from December 1994 to July 1996. Free autogenous iliac bone in the from of particulate cancellous bone and marrow was densely packed into the crib that was adapted to bridge the mandibular discontinuity defect. Frozen-treated autogenous mandibular bone, splitted autogenous rib, and titanium mesh(Dumbach, Leibinger) were used as cribs carrying the PCBM. All ten cases underwent successful healing with the formation of a continuous bony union with the remaining mandible. The rate of resorption was assessed by sequential panoramic radiographs. The mean horizontal dimension of the madibular defects was 44mm and the mean vertical dimension of the reconstructed segments was 23mm. The bony height of the reconstructed segments retained about 90% of the bony height of over a 1-year period. We confirmed that PCBM grafts were the most successful and predictable grafts in mandibular discontinuity reconstruction.