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미생물,발효,생물공학 : Auxin과 항진균물질을 생산하는 식물생장촉진근권세균의 분리동정 및 특성
권도형 ( Kwon Do Hyeong ),최준형 ( Choe Jun Hyeong ),정희경 ( Jeong Hui Gyeong ),임종희 ( Im Jong Hui ),주길재 ( Ju Gil Jae ),김상달 ( Kim Sang Dal ) 한국응용생명화학회 2004 Applied Biological Chemistry (Appl Biol Chem) Vol.47 No.1
This study was investigated the physiological properties of auxin-producing bacteria that have plant growth promoting activity and plant pathogen antagonistic ability. Auxin-producing bacteria were isolated from field soils of Gyeongsan, Korea. Selected strains were identified as a Pseudomonas fulva N2l and a Pantoea agglomerans K35 by morphological and physiological test, and Biolog (Microlog) system. Auxins were determined by Salkowski in vitro test and mungbean adventitious root induction bioassay. Also produced indole-3-acetic acid (IAA) was identified by TLC. During cell growth, auxin production were highest in their idiophase after log phase and 35℃ at pH 7.5.
미생물,발효,생물공학 : 야생꿩으로부터 가금티프스 억제균 Sphingomonas sanguis의 선발 및 항 Salmonella 물질 생산 조건
류향선 ( Lyu Hyang Seon ),이현승 ( Lee Hyeon Seung ),임종희 ( Im Jong Hui ),김진락 ( Kim Jin Lag ),김상달 ( Kim Sang Dal ) 한국응용생명화학회 2004 Applied Biological Chemistry (Appl Biol Chem) Vol.47 No.1
The antagonistic microorganisms against Salmonella gallinarum causing fowl typhoid were isolated from the gut of wild pheasant. The isolated L19, L33, L50 strains were showed the characteristics of isolated Gram negative, rods, catalase positive and oxidase negative. Finally, all strains were identified as Sphingomonas sanguis by Biolog^(?) system. The optimal carbon sources of Sphingomonas sanguis L19, L33 and L50 for the these growth were glucose, saccharose, and fructose respectively. But the optimal carbon sources of S. sanguis L19, L33, L50 for the antagonistic material production were maltose, galactose, and saccharose respectively. The optimal nitrogen sources of S. sanguis L19, L33, L50 for the growth were yeast extract, yeast extrat and NH₄H₂PO₄, respectively. But the optimal nitrogen sources of S. sanguis L19, L33 and L50 for the antagonistic material production were (NH₄)₂SO₄, urea, (NH₄)₂S₂O_(8), espectively.