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- 저자 : 이용호(Yong-Ho Lee)･ (검색결과 2 건)
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남부지방 수집종(蒐集種) 차나무(Camellia sinensis L.) 유연관계 분석
제순자(Soon-Ja Je)･,오주성(Ju-Sung Oh)･,전은희(Eun-Hee Jeon)･,이용호(Yong-Ho Lee)･,정영수(Young-Soo Chung)･,정대수(Dae-Soo Chung) 한국차학회 2005 한국차학회지 Vol.11 No.1
The investigation on the morphological and genetic relationship was carried out with leaves of teas(Camellia sinenesis L.) which were collected from 16 southern areas of Korea including one Japanese location: Hadong, Sachun, Gosueng, Namhae, Milyang, Gimhae, Busan, Jinju, Yangsan, Gurye, Suncheon, Boseong, Gangjin, Haenam, Jeju and Shizhuoka(Japan). In the analysis of genetic relationship, RAPD analysis was done with 30 primers purchased at Bioneer company. There were 80 expanded bands totally observed and 58 bands were found to have polymorphism. The size of expanded DNA was between 200bp and 2,000bp. For the analysis of genetic relationship, NTSYS computer program was used and the similarity value among domestic green teas was 0~0.09. With the similarity value matrix, 12 groups were formed as a result of 16 kinds of dendrogram. The similarity value was so high between Sachun and Gosueng, and between Gurye and Suncheon as 0.90. In the cases of Sachun and Gosueng, there was no difference in leaf length, leaf shape, and leaf width (data not shown) indicating the presence of coincidence between RAPD analysis and morphological analysis. However, in the cases of Gurye and Suncheon, morphological characteristics was not coincident with DNA analysis where both collections belong to same group. Using the polymorphic bands which has a distinct presence or absence in RAPD analysis, the SCAR marker was constructed. Among the 5 SCAR markers, only one from RAPD marker #4 showed a single amplification of 868bp indicating low efficiency of transformation rate from RAPD marker to SCAR marker. But removal of all multiple bands in SCAR amplification clearly revealed that SCAR marker is repetitive and reliable in the amplification of polymorphic DNA region.
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부산·경남지역 사찰에서 수집된 차나무(Camellia sinensis L.)잎 형태별 분류
제순자(Je, Soon-Ja)･,이용호(Lee, Yong-Ho)･,오주성(Oh, Ju-Sung3)･,정영수(Chung, Yong-Soo)･,정대수(Chung, Dae-Soo) 한국차학회 2007 한국차학회지 Vol.13 No.1
본 시험은 부산·경남지역 사찰 주변에 자생하고 있는(또는 30년 이상 재배되어 온) 차나무를 중심으로 14개 지역을 선정하여 산지별로 차나무 잎을 채취, 수집하여 잎 형태별 특성을 조사하였다. 차나무 잎의 형태적 특성 중 엽장(葉長) (12.04㎝), 엽폭(葉幅)(4.90㎝), 엽면적(葉面積)(41.33㎝)은 사천 배방사지 차나무 잎이 가장 크게 나타났으며, 가장 작게 나타난 곳은 부산 전등사 차나무가 엽장(6.30㎝)과 엽폭(2.48㎝)이 작으며, 엽면적은 거제 봉곡사(14.80㎝) 차나무가 가장 작게 나타났다. 엽병장(葉柄長)은 밀양의 엄광사지(0.57㎝)와 사천 배방사지(0.57㎝) 차나무 잎이 가장 길게 나타났으며, 거치수(鋸齒數)는 사천 배방사지(30.13개) 차나무였으며, 엽맥수(葉脈數)는 하동 쌍계사(11.27개) 차나무로 가장 많게 나타났다. 생엽중(生葉重)과 건엽중(乾葉重)은 사천 배방사지 차나무가 생엽중(0.81 g), 건엽중(0.34 g)으로 가장 무거웠으며, 가장 작은 지역은 부산 전등사가 생엽중(0.25 g)이며, 건엽중은 전등사와 범어사(0.10 g)가 가장 가볍게 나타났다. 차나무 잎의 형질간 상관관계는 수량성을 나타내는 건엽중은 엽장, 엽폭, 엽면적, 생엽중과 고도의 유의성을 보였으며 0.8이상의 매우 높은 유의차를 보였다. 본 연구 결과 사천 배방사지 차나무가 엽장(葉長), 엽폭(葉幅), 엽면적(葉面積), 엽병장(葉柄長), 거치수(鋸齒數), 생엽무게, 건조무게 등은 전체적으로 가장 크고 무거웠으며, 엽맥수(葉脈數)는 하동 쌍계사 주변의 차나무가 가장 많았다. 자생하는 차나무의 다양한 유전적 변이를 이용하여 유용한 유전자원으로 이용한다면 우량 다수성 계통의 신품종 개발 가능성이 높을 것으로 판단되며, 금후 자생지역별로 생육환경이나 지리적 특성도 검토 분석 한다면 기능성 신품종 개발 보급에 기초 자료로서 활용성이 높을 것으로 판단된다. We collected the tea leaves from the vicinity of 14 Buddhist temple located in Busan and Gyeongnam area where wild tea trees and more than 30 year old tee trees were commonly found. The characteristics of the tea leaves were surveyed. Among those tee leaves from 14 areas, Baebangsaji (Goseong) tea leaves had the highest value of leaf length (12.04 ㎝), leaf width(4.90 cm), and leaf area (41.33 ㎝). The tea leaves of Jeondeungsa (Busan) had the lowest value of leaf length (6.30 ㎝) and leaf width (2.48 ㎝). The tea leaves of Bonggoksa (Geoje) had the lowest value of leaf area (14.80 ㎝). The longest leaf stalk length was Umgwangsaji (Miryang) tea leaves (0.57 ㎝) and Baedangsaji (Goseong) tea leaf (0.57 ㎝). The largest No. of serrate was Baedangsaji (Goseong) tea leaves (30.13) and for leaf vein was Ssanggyesa (Hadong) as a number of 11.27. The heaviest weight of fresh leaf was Baedangsaji (Goseong) tea leaf (0.81 g) and, also, the dry tea leaf of Baedangsaji (Goseong) was the heaviest (0.34 g). The lightest weight of fresh leaf was Jeondeungsa (Busan) tea leaves (0.25 g) and the lightest weight of dry leaf was Jeondeungsa. In this study, leaf length, leaf width, leaf area, leaf stalk length, No. of serrate, weight of fresh leaf and weight of dry leaf value of the leaves collected from Baedangsaji (Goseong) was generally high and The highest value of No. of leaf vein was from Ssanggyesa (Hadong).
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