8주간의 저항성 운동이 쥐 골격근에서 Mustn1 mRNA 발현에 미치는 영향

오승렬 ( Seung Lyul Oh ) 대한스포츠의학회 2011 대한스포츠의학회지 Vol.29 No.2

The aim of this investigation was to determine if resistance exercise improved musculoskeletal embryonic nuclear protein 1 (Mustn1) mRNA expression in skeletal muscle of rat. Thirty-two male Sprague-Dawley rats were separated into sedentary (control group, CON; n=16) and exercise-trained groups (resistance exercise group, REG; n=16). CON and REG subsequently were separated into 4 weeks group (4 weeks CON, 4 weeks REG) and 8 weeks group (8 weeks CON, 8 weeks REG). The rats in the resistance exercise group were trained to climb a 1-m vertical (85 degree incline) ladder with weights secured to their tail, and they climbed the ladder 10 times 3 days per week for 8 weeks progressively. After weeks of exercise, skeletal muscle was taken from the flexor halucis longus. After separating the total ribonucleic acid (RNA) of each group, quantitative polymerase chain reaction was used to analyze RNA quantitatively. After 4 weeks of resistance exercise, Mustn1 mRNA expression increased significantly in REG compared to CON (p<0.001). Additionally, there was a significant increase of Mustn1 mRNA expression in 8 weeks REG compared to 8 weeks CON (p<0.01). Interestingly, there was a significant difference in Mustn1 mRNA between 4 weeks REG and 8 weeks REG (p<0.01). In the REG, Mustn1 mRNA increased by 3.7-fold and 2.1-fold relative to CON, respectively. In conclusion, the resistance training increased Mustn1 mRNA expression in skeletal muscle of rat. This shows that the Mustn1 mRNA expression gives positive effect on myogenesis and muscle regeneration in skeletal muscle of rat results from resistance ladder exercise.

대규모 유전자 발현양상 분석을 통한 저항성 운동수행능력 유전자 발굴

오승렬 ( Seung Lyul Oh ) 한국운동생리학회 2016 운동과학 Vol.25 No.1

PURPOSE: The purpose of this study was to examine changes of gene transcripts following 4- and 8-week resistance exercise training in rat skeletal muscle using large-scale BeadArray containing probe sets for 22,523 genes. METHODS: Forty-eight male Sprague-Dawley rats were enrolled and into control (CON, n=16) and and exercise (resistance training, RT, n=32) groups. After 4- and 8-weeks of resistance exercise, based upon lifting weight of individual rats, RT subsequently was separated into high performance group (H-RT4, n=8; H-RT8, n=8) and low performance group (L-RT4, n=8; L-RT8, n=8). Rats were assigned to RT were trained to climb a 1-m vertical (85 degree incline) ladder with weights secured to their tails, and they performed 1 set of 10 times of 3 days per week for 8 weeks progressively. Skeletal muscle was taken from the flexor halucis longus (FHL) after 4- and 8-weeks exercise. After separating the total RNA of each group, large-scale gene expression was analyzed by BeadArray using Illunina RatRef-12 Expression BeadChip, and qPCR was used to inspect BeadArray data. We selected genes given the criteria that detection p-value of <.01, M-value {M=log2(condition)-log2(reference)} to >1.0, and DiffScore to >30. RESULTS: The expression of 20 genes significantly increased 4 weeks after resistance exercise compared to L-RT4, and the expression of four genes decreased, including genes with unknown functions. At 8 weeks, the expression of one gene significantly increased compared to L-RT8 and that of one decreased. These genes involved in acute phase response (Hp, Ahsg), anti-inflammatory response (Ambp), C21-steroid hormone biosynthesis (Timp1), heme oxidation (Hmox1), muscle development (Myh6), regulation of cell growth (Igfbp1), vitamin D metabolism (Gc), calcium-mediated signaling (Dscr1), gluconeogenesis (Atf3), regulation of transcription (Nr4a3), and learning and memory (Egr1). CONCLUSIONS: This is the first study to use BeadArray to examine the global mRNA expression in rat skeletal muscle after resistance ladder training. We found expression genes of rat skeletal muscle based on the resistance exercise performance level using BeadArray. These results may help to explain previously reported physiological changes of skeletal muscle and suggest new avenues for further investigation.

규칙적인 저항성 운동이 IGF-1과 UCP-3 mRNA 발현에 미치는 영향

오승렬 ( Seung Lyul Oh ),황인근 ( In Geun Hwang ),오상덕 ( Sang Duk Oh ) 대한스포츠의학회 2009 대한스포츠의학회지 Vol.27 No.2

This research is about the effect of 4 weeks` resistive exercise on IGF-1 and UCP-3, where they are known for major muscle effecting genes and having effects on tissue growth & development, and body metabolism. Twenty-four male Sprague-Dawley rats were included at 10 wks of age and assigned into control group (CG) and resistance exercise group (EG). After 4-weeks of resistance exercise, the total RNA of each group was separated. And qPCR was used to analyze RNA quantitatively. After 4-weeks of regulated resistance exercise, there was statistical difference in IGF-1 and UCP-3 mRNA expression (p<0.001) in skeleton muscles between the control group and the resistance exercise group. At post-hoc, EG had significantly higher IGF-1 and UCP-3 mRNA expression than CG (p<0.01; p<0.001) and the 4th week CG (p<0.001; p<0.001). In conclusion, the resistance exercise increased IGF-1 mRNA expression in the skeleton muscle, and after the experiment there was significant increase in UCP-3 mRNA expression as well.

Effect of Resistance Training on Skeletal Muscle Gene Expression in Rats

Seung-Lyul Oh(오승렬),Sang-Duk Oh(오상덕) 한국생명과학회 2013 생명과학회지 Vol.23 No.1

본 연구의 목적은 저항성 운동 후 골격근에서 저항성 관련 유전자를 규명하는 것이다. 연구 목적을 달성하기 위하여 총 32두의 Sprague-Dawley계 수컷 흰쥐를 분양 받은 후 4주차 통제군(4 wks CON, n=8), 8주차 통제군(8 wks CON, n=8), 4주차 운동군(4 wks REG, n=8), 8주차 운동군(8 wks REG, n=8)으로 집단을 분류하였다. 저항성 운동군은 꼬리에 무게를 달고 동물용 사다리(1-m vertical, 85 degree incline)를 오르는 저항성 사다리 운동을 1회 10번, 주당 3일, 4주와 8주간 점증적으로 실시하였으며, 골격근 조직은 저항성 운동 후 장무지굴근(flexor hallucis longus; FHL)을 적출하여 분석에 이용하였다. 적출한 골격근에서 total RNA를 분류한 후, 대규모 유전자 발현분석을 위하여 Illumina RatRef-12 Expression BeadChip을 이용한 Beadarray를 시행하였으며, Beadarray 결과를 확인하기 위해 qPCR (real-time quantitative PCR)를 실시하였다. 유의성 검증은 Beadstudio software를 이용하여 실시하였으며, Beadarray 데이터 중 Detection p-value to <0.01, M-value {M= log2 (condition)-log2 (reference)} to >1.0, DiffScore to >20인 유전자만을 통계적으로 의미 있는 유전자로 선택하였다. 4주차 저항성 운동 후 통제집단에 비해 2배 이상 유의하게 발현이 증가한 유전자는 30개였으며, 6개의 유전자가 감소하였다. 8주차 저항성 운동 후에는 5개의 유전자가 발현이 증가하였으며, 12개의 유전자가 유의하게 감소하였다. 연구결과 다음의 유전자를 포함한 저항성 운동과 근비대와 관련 후보 유전자를 도출하였다; 1) 세포 성장 조절(IGFBP1, PLA2G2A, OKL38); 2) 근육발생(CSRP3); 3) 조직 재생과 근육 발달(MUSTN1, MYBPH); and 4) 비대 모델(CYR61, ATF3, NR4A3); and 5) 당대사(G6PC, PCK1). 이러한 연구결과는 차후 저항성 운동과 관련된 다양한 생리학적 변인을 연구하는데 있어서 기초 자료를 제공할 것으로 생각된다. The aim was to examine resistance exercise-related genes after 8 weeks of resistance training. Thirty-two male Sprague-Dawley rats were divided into four groups: 4 weeks sedentary (4 wks CON, n=8), 8 weeks sedentary (8 wks CON, n=8), 4 weeks exercise training (4 wks REG, n=8), and 8 weeks exercise training (8 wks REG, n=8). The rats were trained to climb a 1-m vertical incline (85-degree), with weights secured to their tails. They climbed 10 times, 3 days per week, for 8 consecutive weeks. Skeletal muscle was taken from the flexor halucis longus after the exercise training. After separating the total RNA, large-scale gene expression was investigated by beadarray (Illumina RatRef-12 Expression BeadChip) analysis, and qPCR was used to inspect the beadarray data and to analyze the RNA quantitatively. The detection p-value for the genes was p<0.01, the M-value {M=log₂(condition)-log₂(reference)} was >1.0, and the DiffScore was >20. In total, the expression of 30 genes significantly increased 4 weeks after the exercise training, and the expression of six genes decreased. At 8 weeks, the expression of five genes significantly increased and that of 12 decreased. Several genes are potentially involved in resistance exercise and muscle hypertrophy, including 1) regulation of cell growth (IGFBP1, PLA2G2A, OKL38); 2) myogenesis (CSRP3); 3) tissue regeneration and muscle development (MUSTN1, MYBPH); 4) hypertrophy (CYR61, ATF3, NR4A3); and 5) glucose metabolism (G6PC, PCK1). These results may help to explain previously reported physiological changes of the skeletal muscle and suggest new avenues for further investigation.

8주간의 비교적 빠른 각속도에 따른 등속성 운동 프로그램이 등속성 근력 향상에 미치는 효과

오승렬 ( Seung Lyul Oh ),오상덕 ( Sang Duk Oh ) 한국스포츠리서치 2007 한국 스포츠 리서치 Vol.18 No.4

The purpose of the study was to apply isokinetic exercise programs of 180deg/sec, 240deg/sec and 300deg/sec during 8-weeks by each group and to investigate test results of muscular strength before and after exercise and to verify effects of isokinetic exercise programs of quick angular velocity. The subjects were 30 college students who attended ``H`` University. The subjects were divided into three groups having each 10 students depending upon angular velocity. Two-way ANOVA with repeated measurements was used to investigate difference of isokinetic exercise effects between groups, and paired samples t-test was used to investigate significance of changes of knee joint muscular strength before and after isokinetic exercise of each group. The findings were as follow; Firstly, changes of muscular strength of knee joints had significant difference at both extensor and flexor before and after exercise that was variables of the group(p<.001). The muscular strength improvements of 180deg/sec, 240deg/sec and 300deg/sec group had no significant difference at both extensor and flexor. Interaction between subjects groups and within subjects conditions had no significant difference; Secondly, the muscular strength of knee joint was improved after isokinetic exercise of knee joint of each group: 180deg/sec group had significant increase of left-hand flexor (p<.05). 240deg/sec group had significant increase of right-hand extensor as well as left-hand extensor (p<.001, p<.01). 300deg/sec group had significant increase of both extensor and right-hand flexor (p<.05, p<.01, p<.01). In conclusion, isokinetic exercise program having quick velocity was partially effective to improve muscle strength, and 180deg/sec, 240deg/sec and 300deg/sec groups that had relatively high velocity had no significant difference. Therefore, not only low velocity isokinetic exercise but also high velocity isokinetic exercise should reorganize programs to make isokinetic exercise program for effective improvement of muscular strength.

ACTN3 유전자 Q523R 다형성과 운동수행능력

오승렬(Oh Seung-Lyul),오상덕(Oh Sang-Duk) 한국체육과학회 2011 한국체육과학회지 Vol.20 No.1

This study investigated the association between ACTN3 Q523R genotype and exercise performance in the Korean non-athletes [n=38, 38 men, age: 20~21 years]. We hypothesized that the Q allele might be associated with greater muscular strength and power, whereas R allele might be associated with higher aerobic endurance. We were measured isokinetically (at 60, 180 and 300oS-1), anaerobic wingate test, and graded exercise test. Total genomic DNA was isolated from antecubital vein of subjects, and ACTN3 Q523R genotype was determined by polymerase chain reaction. This study suggests that the presence or absence of α-actinin-3 significantly influence the isokinetic strength (extensor; p<.01, flexor; p<.001) and power (extensor; p<.001, flexor; p<.05), anaerobic power ( p<.05), and aerobic endurance (VO₂max; p<.05, exercise time; p<.001). In conclusion, this study suggests that the absence of α-actinin-3 is detrimental to anaerobic power performance in the Korean non-athletes. In contrast, α-actinin-3 deficiency appears to be beneficial for isokinetic strength, power, and aerobic endurance performance. And homozygote for the Q allele was negatively associated with isokinetic strength and power performance.

높은 지도 비율의 전신진동운동이 비만 중년여성의 신체조성, 기초체력, 혈중지질에 미치는 영향

오승렬(Oh, Seung-Lyul) 한국체육과학회 2019 한국체육과학회지 Vol.28 No.2

The purpose of this study to examine the effect of 8 weeks of whole body vibration exercise (WBVE) on body composition, physical fitness and blood lipid profiles in middle-aged overweight women. We recruited overweight participants (over 45 years, BMI > 25 kg/㎡, or % body fat > 32%, n=16), and randomly assigned them to either the control group (Con, n=8) or the exercise group (Ex, n=8). All participants underwent the evaluation for body composition, physical fitness and blood lipid profiles before and 8 weeks after the interventions. The Ex group performed the WBVE for 40 min/day, 3 days a week for 8 weeks. The WBVE comprised of standing, squat, heel rise, and push-up positions, and the frequency and amplitude are controlled for an incremental overload exercise. The interaction effect was significantly different in the health-related fitness (muscle strength and endurance, cardiovascular endurance, flexibility, and percent of body fat) and the skill-related fitness such as agility and single-leg balance after 8 weeks of intervention relative to the baseline of the Con and Ex groups. In addition, the WBVE for 8 weeks significantly increased HDL-cholesterol and decreased body weight. These results suggest that WBVE program induces improvement of physical fitness and obese factors in middle-aged overweight women. Therefore, the WBVE might be an effective exercise method to promote physical fitness and weight loss in middle-aged overweight women.

유산소성 운동 강도가 혈중 알코올 농도에 미치는 영향

오승렬,오상덕,김태욱,박원화 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

This study was to examine the most effective exercise intensity to decrease blood alcohol level under the condition of the same exercise volume after alcohol ingestion The subjects of the study were 7 students who enrolled in physical education major at H University, The blood alcohol levels of subjects were recorded and analyzed in different exercise condition, which were without exercise, HRmax 50% aerobic exercise, HRmax 70% aerobic exercise at each exercise situation(before, immediately after, 65rnins after, and 120mins after exercise). The effects of aerobic exercise on blood alcohol level and the changes in blood alcohol concentration level according to aerobic exercise intensity were as follows : 1. The blood alcohol levels in non-exercise group, HRmax 50% exercise group, and HRmax 70% exercise group illustrated significant difference(p<.05), In post hoc test, there was significant difference between non-exercise group and HRmax 50% exercise group (p<.05). In addition, there was significant difference between non-exercise group and HRmax 70% exercise group(p<.05). However, the blood alcohol levels in HRmax 50% exercise group and HRmax 70% exercise group did not show significant difference(p>.05). 2. The levels of decreasing blood alcohol concentration in the exercise situation(before, immediately after, 60mins after, and 120mins after exercise) showed significant difference(p<.001). 3. The interactive effect between exercise group and exercise situation illustrated significant difference(p<.001). As mentioned above, aerobic exercise was effective to decrease blood alcohol level after alcohol ingestion but under the condition of the same exercise volume the change of blood alcohol levels according to exercise intensity did not show significant difference. In conclusion, the most effective and fastest way to decrease blood alcohol level was HRmax 50% aerobic exercise, that is low intensity, rest after the exercise, and slight physical activity.