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In vitro bioassay for estrogen-like endocrine disrupting chemicals
ICHIKAWA, Kazuhiro,HAN, Sang-Kook,KIM, Su-Hyon,UTSUMI, Hideo 嶺南大學校 環境問題硏究所 1998 環境硏究 Vol.18 No.1
Eighty chemicals including pesticides synthetic estrogens, raw materials used in industry were applied to E-Screen assay in this study. MCF-7 cell, used in E-Screen assay, is known to be proliferated by addition of estrogens and have been widely used to defect estrogen-like chemicals. Nine of 80 chemicals showed proliferative activity in a dose-dependent manner. Six of these 9 chemicals were hsted as EDCs in Strategic Programs on Environmental Endocrine Disruptors by Environmental Agency. Government of Japan Four chemicals were recognized to have proliferative activities in this study.