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RISS 인기검색어
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- 저자 : 송우근 ( Man Sik Kang (검색결과 2 건)
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강만식,이정주,송우근,Kang, Man-Sik,Lee, Chung-Choo,Song, Woo-Keun 생화학분자생물학회 1984 한국생화학회지 Vol.17 No.2
In order to study the cell fusion mechanism in chick embryo muscle cells in culture, the changes of cell surface proteins and the effect of FGF during myogenesis were analyzed by using lactoperoxidase-catalyzed iodination and purified FGF addition to the culture medium, respectively. Both quantitative and qualitative changes were observed in $^{131}I$-labelled surface proteins during myogenesis. The proteins with molecular weight of 245K and 66K decreased as the fusion proceeded, and 130K and 93K proteins appeared in the course of fusion. The 245K protein seems to be the LETS protein, and the appearance of 130K and 93K proteins may be due to proteolytic cleavage of the 245K protein. At the same time, four labelled proteins were detected in the cultured medium, which seem to be released from the membrane surface proteins by proteolysis in the course of myoblast fusion. In order to examine the effect of mitogen on myoblast fusion, FGF was partially purified from bovine brain. When FGF was added to culture medium, myoblast exhibited an increase in DNA synthesis and the delay in fusion. These results suggest the possibility that the onset of myoblast fusion is due to the depletion of replication·promoting components in culture medium. The changes of cell surface protein, the engagement of these proteins in myoblast fusion, and the effect of environmental factors on myoblast fusion were discussed. 세포의 융합기작의 일단을 규명하기 위하여 계배 근원세포의 막표면 단백질을 lactoperoxidase를 써서 iodination하여 융합 전후의 막단백질의 변화를 분석하였고, fibroblast growth factor(FGF)를 소의 뇌로부터 추출하여 근원세포의 DNA 함성과 융합에 미치는 영향을 검토하였다. 융합과정 중에 막표면 단백질의 정성적 및 정량적 변화를 볼 수 있었는데, 융합이 진행됨에 따라 245K 와 66K 단백질은 점차 감소하였고 130K와 93K 단백질은 새로 출현하였다. 245K 단백질은 large, external, transformation-sensitive 단백질로 추정되며, 이 단백질의 가수분해 결과 130K와 93K 단백질이 출현하는 것으로 추정되었다. 또한 배양액 내에서 발견된 4개의 표지단백질도 막단백질의 가수분해 산물로 생각되었다. FGF를 배양액에 첨가하였을 경우 근원세포의 DNA 합성률은 증가를 보인 반면 융합은 지연되는 현상을 관찰할 수 있었다. 이러한 사실은 근원세포의 융합이 배양액 내에 존재하는 DNA 합성을 촉진하는 물질이 고갈됨에 따라 개시되는 것이 아닌가 하는 점을 암시해 주는 것으로 사료되었다. 근원세포의 융합과정에서 나타나는 막표면 단백질의 변화와 외적 요인이 세포융합에 미치는 영향에 대해 논의하였다.
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계배근원세포의 분화에서 막표면단백질의 변화와 섬유아제포성장인자의 작용
강만식,이정주,송우근 ( Man Sik Kang,Chung Choo Lee,Woo Keun Song ) 생화학분자생물학회 1984 BMB Reports Vol.17 No.2
In order to study the cell fusion mechanism in chick embryo muscle cells in culture, the changes of cell surface proteins and the effect of FGF during myogenesis were analyzed by using lactoperoxidase-catalyzed iodination and purified FGF addition to the culture medium, respectively. Both quantitative and qualitative changes were observed in ^(131)I-labelled surface proteins during myogenesis. The proteins with molecular weight of 245K and 66K decreased as the fusion proceeded, and 130K and 93K proteins appeared in the course of fusion. The 245K protein seems to be the LETS protein, and the appearance of 130K and 93K proteins may be due to proteolytic cleavage of the 245K protein. At the same time, four labelled proteins were detected in the cultured medium, which seem to be released from the membrane surface proteins by proteolysis in the course of myoblast fusion. In order to examine the effect of mitogen on myoblast fusion, FGF was partially purified from bovine brain. When FGF was added to culture medium, myoblast exhibited an increase in DNA synthesis and the delay in fusion. These results suggest the possibility that the onset of myoblast fusion is due to the depletion of replication-promoting components in culture medium. The changes of cell surface protein, the engagement of these proteins in myoblast fusion, and the effect of environmental factors on myoblast fusion were discussed.
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