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RISS 인기검색어
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- 저자 : 서무규 ( Seo Mu Gyu ) (검색결과 2 건)
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흑색진균에서 중합효소 연쇄반응 및 제한효소 절편길이 다형성을 이용한 Ribosomal DNA 분석
서무규 ( Seo Mu Gyu ),김연진 ( Kim Yeon Jin ),권순욱 ( Kwon Sun Ug ),김정철 ( Kim Jeong Cheol ) 대한피부과학회 2003 대한피부과학회지 Vol.41 No.11
N/A Background : There are three kinds of diseases caused by dematiaceous fungi : chromoblastomycosis, phaeohyphomycosis, and eumycotic mycetoma. The dematiaceous fungi have been identified and classified by morphological, biochemical and physiological tests. Recently molecular analysis has been introduced to the field of medical mycology. Objective : Ribosomal DNA gene analysis of dematiaceous fungi using polymerase chain reaction(PCR)-restriction fragment length polymorphism(RFLP) is investigated. Methods : The dematiaceous fungal strains studied were eight clinical isolates of chromoblastomycosis and phaeohyphomycosis agents(3 strains of Fonsecaea pedrosoi, 2 strains of Exophiala dermatitidis, 1 strain of Exophiala jeanselmei, 1 strain of Phialophora verrucosa, 1 strain of Rhinocladiella aquaspersa) and 4 standard strains(F. pedrosoi IFM 4889, E. dermatitidis IFM 4828, P. verrucosa IFM 4928, R. aquaspersa IFM 4930). Total twelve strains of dematiaceous fungi were cultured on Sabouraud dextrose broth and their DNA was extracted by bead-beating method. PCR-RFLP of ribosomal gene small subunit(SSU rDNA) and internal transcribed spacer(ITS)1-ITS2 ribosomal regions using the primer pairs NS1-NS2 and ITS1-ITS4, respectively were done. Results : The PCR product obtained from the SSU rDNA amplification was of approximately 603 bp and restriction profile was clustered into two genetically heterogenous groups, the first one formed by F. pedrosoi and the second one formed by other dematiaceous fungi. In contrast, the amplifiedb PCR products of ITS regions ranged in sized from 580 to 620 bp and restriction profile was clustered into five genetically heterogenous groups and it can be possible to differentiate dematiaceous fungi. But one clinical isolate of F. pedrosoi showed intra-specific variability. Conclusion : The PCR-RFLP analysis of SSU rDNA and ITS regions provided useful information for identification and classification of dematiaceous fungi. (Korean J Dermatol 2003;41(11) : 1478∼1486)
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조갑진균증의 임상 양상 및 원인균 동정 (1999-2002)
임성욱 ( Im Seong Ug ),서무규 ( Seo Mu Gyu ),하경임 ( Ha Gyeong Im ) 대한피부과학회 2004 大韓皮膚科學會誌 Vol.42 No.1
N/A Background: Although there have been many studies about tinea unguium, few studies about etiologic agents including nondermatophytic molds and yeasts in onychomycosis have been reported in Korea. Objective: The purpose of the study was to investigate the recent clinical features and identification of etiologic agents in onychomycosis. Methods: In the 3-year period 1999-2002, we reviewed five hundred ninty nine patients with onychomycosis in retrospectively. The etiologic agents were identified by cultures on Sabouraud`s dextrose agar with and without cycloheximide. The identification of yeasts based on the results of culture, germ tube test, and biochemical API system tests. Nondermatophytic molds or yeasts isolated were considered as pathogens when the presence of fungal elements was identified at direct microscopy and follow-up specimen yielding cultures showed the same fungi. Results: Of the five hundred ninty nine patients presenting with onychomycosis, 92.5% were toenail onychomycosis, 5.5% fingernail onychomycosis, and 2.0% onychomycosis in both toenails and fingernails. Among the age groups, the incidence rate was highest in the fifth decade(22.0%). The ratio of male to female patients was 1.1:1. Distal subungual onychomycosis(96.1%) was the most common clinical type of onychomycosis. In the toenail onychomycosis, dermatophytes were most frequently isolated(81.9%), followed by yeasts(11.7%), and nondermatophytic molds(6.4%). Tricho-phyton rubrum was the most frequently isolated agent. In the fingemail onychomycosis, yeasts were mostly isolated(48.2%), followed by dermatophytes(44.4%), and nondermatophytic molds(7.4%). Conclusion: Because of the increase in onychomycosis by nondermatophytic molds and yeasts, we suggest the need of a careful mycological examination in patients with onychomycosis. (Korean J Dermatol 2004;42(1):53~60)
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