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감자 ( Solanum tuberosum ) 의 기내 소괴경 형성 단계에 따른 Peroxidase 와 Esterase 동위효소의 양상 변화
정현숙(Hyeon Sook Cheong),송규상(Kyu Sang Song),박진수(Gene Su Park),김성준(Sung Jun Kim),양영기(Young Ki Yang),표병식(Byoung Sik Pyo),황백(Baik Hwang),강영희(Young Hee Kang) 한국식물학회 1993 Journal of Plant Biology Vol.36 No.1
The microtuber was efficiently formed on SH medium containing 9% sucrose from the in vitro propagated shoot of potato (Solanum tuberosum cv. Sumi). In order to investigate gene expression depending on the developmental stage of microtuber, we examined the changes of peroxidase and esterase activities, and their isozyme patterns as well. Peroxidase and esterase activities were the highest at the 7 day-culture of the microtuber and subsequently decreased on the stage of microtuberization, whereas esterase activity increased at the stage of 60 day-culture. However, their activities in the ordinary tuber were higher than those of 60 day-cultured microtuber. In addition, in the peroxidase isozyme pattern two new bands of pI 7.05 and pI 4.65 were appeared at the 15- day and 60 day-cultures, respectively, as shown by isoelectric focusing. Various bands in the esterase isozyme pattern were shown at the 7 day-culture, and the band patterns were a large difference, comparing those of shoot and tuber. New bands in the esterase isozyme pattern also appeared at the 15 day- (pI 4.52) and 60 day-cultures (pI 4.48). These results suggest that the changes of peroxidase and esterase activities and isozyme patterns are an important factor in the differentiation and development of potato.