http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
고들빼기(Ixeris sonchifolia H.) 성분 및 생물학적 활성에 관한 연구(I)
박수선,Park, Soo-Sun 생화학분자생물학회 1977 한국생화학회지 Vol.10 No.4
식용 및 약용으로 쓰이는 우리나라 야생 식물 고들빼기(Ixeris sonchifolia Hance)의 성분을 분석하여 chlorogenic acid, 7종의 필수 아미노산 및 16종의 총 아미노산을 확인하였다. 아미노산중 proline의 함량이 가장 많았다. 고들빼기의 엑기스는 Ehrlich암에 대해 항암작용을 나타내었다. In Ixeris Sonchifolia H., which is an edible and medicinal plant in Korea chlorgenic acid was identified by P.P.C. and T.L.C. Sixteen total amino acids (Asp, Thr, Ser, Glu, Pro, Gly, Ala, Val, Met, Ile, Leu, Tyr, Phe, Lys, His, Arg) including seven essential amino acids except tryptophan were identified in Ixeris Sonchifolia H. Of these sixteen amino acids, especially proline was contained in the highest amount. The extract of the plant showed an antineoplastic action on Ehrlich carcinoma.
한국산 재래종 담배잎에서 정제한 Polyphenol Oxidase의 성상에 관한 연구
박수선(Soo Sun Park),김안근(An Geun Kim),박금영(Geum Young Park) 한국생약학회 1989 생약학회지 Vol.20 No.2
Purification of polyphenol oxidase(PPO) from Korean native tobacco variety leaves was carried out through the procedure of acetone preciptation, ammonium sulfate fractionation, and Sephadex G-150 gel filtration, resulting in a 84-fold increase in specific activity. The enzyme was stable in a range of pH 7.5 to 8.0 with an optimum of pH 7.5, The optimum temperature for the enzymic reaction was about 60°. It was thermostable with a half-life equal to 20 min at 70°. Km values for (+)-catechin and pyrogallol were 1.6×10^(-3) and 0.5×10^(-3) M, respectively. It possesses high catecholase activity but little or no cresolase activity. Lineweaver-Burk analysis of inhibition data revealed that the inhibition of (+)-catechin oxidation by potassium cyanide, 4-nitrocatechol, cystein and 2-mercaptoethanol was competitive with Ki values of 1.1×10<sup>-6</sup>, 1.8×10<sup>-6</sup>, 8.9×10<sup>-6</sup> and 1.3×10^(-5) M, respectively.
박수선(Soo Sun Park),김안근(An Keun Kim) 한국생약학회 1984 생약학회지 Vol.15 No.2
Polyphenol oxidase was purified from acetone powder extract of the root of Ixeris sonchifolia. The enzyme obtained by ammonium sulfate fractionation and sephadex G-200 gelfiltration gave 51-fold purification over the crude extract. The purified enzyme showed activity toward chlorogenic acid, caffeic acid and pyrocatechol. The kinetics of thermal inactivation of the enzyme followed first-order reaction. Potassium cyanide and cysteine were potent inhibitors.
아미노산류가 들깨잎 폴리페놀 옥시다제 활성저해에 미치는 영향
박수선(Soo Sun Park),김안근(An Keun Kim),손은수(Eun Soo Sohn) 대한약학회 1996 약학회지 Vol.40 No.1
Characterization of Polyphenol oxidase (PPO) in Perillae Folium, particullarly inhibitor studies were investigated. This enzyme was stable at pH 5.0 and the residual activity of PPO at >or= ph 5.5 was estimated to be very low. PPO activity was decreased slightly by adding amino acid with catechol as a substrate, particullary PPO activity was inhibited markedly by cystein, histidine, lysine and arginine. In the absorption spectra of the product formed when catechol was oxidized by PPO, with a ramdamax at 410nm, the peak shifted toward ramdamax 520nm by addition of L-proline. At relatively low concentrations(10-3M), sulfite and dithiothreithol completely inhibited PPO activity. Inhibition of PPO activity by amino acids and inhibitors increased or decreased depending on the pH used to measure it
고등 식물중의 Polyphenol 성분에 관한 연구(Ⅳ)
박수선(Soo Sun Park),한정순(Jung Soon Han) 한국생약학회 1975 생약학회지 Vol.6 No.2
Polyphenol substances involved in the browning of Malus asiatica NAKAI (Hong-ok) were examined. It was found that chlorogenic acid was the principal substance. The estimation of polyphenol oxidase activity in Malus asiatica revealed that its browning reaction was caused by enzymatic oxidation of chlorogenic acid.
갈근 중의 Polyphenol Oxidase에 관한연구
박수선(Soo Sun Park),김안근(An Keun Kim),이정신(Jeong Sin Lee) 한국생약학회 1991 생약학회지 Vol.22 No.2
Polyphenol oxidase(PPO) was purified from an extract of Puerariae Radix by ammonium sulfate fractionation followed by Sephadex G-150 column chromatography, which resulted in a 56-fold increase in specific activity. The enzyme was optimum of pH 6.5. The optimum temperature of enzymic reaction was about 40° The enzyme was thermostable with a half-life equal to 32 min at 70°. Km values of the PPO for catechol and pyrogallol from Lineweaver Burk plots were 1.3×10^(-2) M, 1.16×10^(-2) M, respectively. The substrate specificity of the Puerariae Radix PPO showed high affinity toward pyrogallol. Reducing reagents such as cysteine, potassium metabisulfite, ascorbic acid, 2-mercaptoethanol completely inhibited the PPO activity at 10^(-2)M level. Linewear-Burk analysis of inhibition data revealed that the inhibition by cysteine, 2-mercaptoethanol, 4-nitrocatechol, potassium cyanide was competitive with Ki values of 4.3×10^(-2) M, 0.73×10<sup>-6</sup> M, 6.9×10<sup>-6</sup> M, 6.4×10^(-7) M, respectively. The browning reaction by PPO was observed to decrease temporarily with the addition of sodium diethyl dithiocarbamate, a well known copper chelating agent. Among the divalent cations, Cu^(2+) ion was strong activator on PPO and Mn^(2+), Co^(2+) ions was effect on PPO activity. Zn^(2+), Mg^(2+) ions was inhibitor on PPO.
박수선(Soo Sun Park),김안근(An Keun Kim),노진희(Jin Hee Roh),심미옥(Mi Ok Shim) 대한약학회 1991 약학회지 Vol.35 No.3
Polyphenol oxidase (PPO) was purified from an extract of Perillae Folium by ammonium sulfate fractionation and sephadex G-150 gel filtration, which molecular weight estimated 65,000+/-1,000 in SDS-gel electrophoresis, and pI value was 4.8. The pH and temperature optima were 6.0 and 30oC respectively. Km values of the PPO for various phenolics derived from Lineweaver-Burk plots were 4.OX1O-4, caffeic acid; 4.2XlO-3M, 4-methylcatechol. The inhibition by 4-nitrocatechol, potassium cyanide, cysteine, 2-mercaptoethanol was competitive with K, values of 7.6X10-5 M, 7.2X10-5M, 3.6X10-5M, 2.2X10-5M, respectively. Among the divalent cations, Cu2+ ion was strong activator on PPO and Zn2+ ,Ni2+ ions were little effect on PPO activity. In comparing the amino acid composition of Perillae Folium PPO with that of wheat isozyme, grape, spinach showed similarity. But the content of glycine phenylalanine was abundant relatively.
고들빼기 ( Ixeris sonchifolia H . ) 성분 및 생물학적 활성에 관한 연구 ( 1 )
박수선 ( Soo Sun Park ) 생화학분자생물학회 1977 BMB Reports Vol.10 No.4
In Ixeris Sonchifolia H., which is an edible and medicinal plant in Korea chlorgenic acid was identified by P. P. C. and T. L. C. Sixteen total amino acids (Asp, Thr, Ser, Glu, Pro, Gly, Ala, Val, Met, Ile, Leu, Tyr, Phe, Lys, His, Arg) including seven essential amino acids except fiyptophan were identified in Lxeris Sonchifclia H. Of these sixteen amino acids, especially proline was contained in the highest amount. The extract of the plant showed an antineoplastic action on Ehrlich carcinoma.
고등식물중의 Polyphenol 성분에 관한 연구 (Ⅲ)
박수선(Soo Sun Park),김경순(Kyung Soon Kim) 한국생약학회 1973 생약학회지 Vol.4 No.4
In Cnidium officinale MAKINO and Platycodon grandiflorum A. DE CANDOLE, chlorogenic acid was identified by Rf values, color reactions on paper chromatograms and UV-absorption spectra of the eluate of phenolic spots. And isochlorogeni acid-like substance was also found in the former. l-Phenylalanine-U-C<sup>14</sup> and sodium acetate-2-C<sup>14</sup> werse fed to both plants and their incorporation ratio to chlorogenic acid and isochlorogenic acid-like substance was compared, Phenylalanine was better precursor for chlorogenic acid in both plants than acetate. But acetate showed higher incorporation ratio to isochlorogenic acid-like substance in Cnidium officinale than that of phenylalanine.