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발효, 세포배양, 생물공정 Rhodobacter capsulatus hemA 유전자 발현 대장균에 의한 5-Aminolevulinic Acid 생산의 최적화
양동수 ( Dong Soo Yang ),박문원 ( Moon Won Park ),임수진 ( Soo Jin Lim ),김민정 ( Min Jeong Kim ),신유리 ( Yu Ri Shin ),박찬수 ( Chan Soo Park ),현영 ( Young Hyun ),강대경 ( Dae Kyung Kang ) 한국미생물생명공학회 2009 한국미생물·생명공학회지 Vol.37 No.2
Recombinant Escherichia coli BLR(DE3) harboring the hemA gene from Rhodobacter capsulatus under the control of a constitutive promoter, which we constructed previously, was used for the extracellular production of 5-aminolevulinic acid (ALA). The effects of several factors on ALA production were investigated in flask culture. ALA production by the recombinant E. coli was more efficient at 30℃ than 37℃. The glycine concentration had an important effect on cell growth. Glycine and succinic acid concentration of 5-10 and 10-20 g/L, respectively, resulted in high ALA production. In addition, the partial replacement of succinic acid by sodium glutamate increased the ALA production. The ALA production was inhibited by the presence of glucose in the medium. Using the optimal conditions, an ALA concentration of 8.2 g/L was achieved in jar fermentation without an added inducer or ALA dehydratase inhibitor; this is the highest reported concentration.