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Construction and integration of soybean RFLP genetic linkage maps
이석하(Suk Ha Lee),박금룡(Keum Ryong Park),유용환(Yong Hwan Ryu),김석동(Seok Dong Kim) 한국육종학회 1997 한국육종학회지 Vol.29 No.3
Two F₂ soybean (Glycine max L.) populations, Suwon 157 x Danbaekkong’ and ‘Pureunkong’ x ‘Jinpumkong 2’, were evaluated with restriction fragment length polymorphism (RFLP) markers to construct RFLP genetic linkage maps. For the Suwon 157 and Danbaekkong population, 107 F₂-derived lines were scored for segregation at 97 RFLP loci. Molecular-marker analysis with Mapmaker computer program revealed that the genetic map consisted of 72 RFLP loci which converged into 18 linkage groups and covered more than 600 centimorgan (cM). For the Pureunkong and Jinpumkong 2 population, 100 F₂-derived lines were evaluated for segregation at 92 loci. The genetic linkage map of these 74 loci covered about 700 cM and converged into 20 linkage groups. Two idependent maps were integrated with Joinmap computer package on the basis of 35 RFLP markers which were common to both maps. The integrated map covered about 950 cM with 124 markers classified into 24 linkage groups. As four parental soybean genotypes from both populations have been used to make traditional soybean food products such as tofu, sobean milk, and soybean sprouts, this integrated map will provide useful information on the map position of genes which are associated with special food processing traits in soybean.