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쏙(Upogebia major) 효소가수분해물의 면역증강 효과
이지현 ( Ji-hyun Lee ),양지은 ( Ji-eun Yang ),송재희 ( Jae-hee Song ),맹상현 ( Sang-hyun Maeng ),김소연 ( So-yeon Kim ),윤나영 ( Na-young Yoon ) 한국수산과학회(구 한국수산학회) 2018 한국수산과학회지 Vol.51 No.2
This study investigated the immuno-potentiating activities of Japanese mud shrimp Upogebia major. We examined the effects of enzymatic hydrolysate from U. major on the production of nitric oxide (NO) and prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) and on the expression of pro-inflammation cytokines including TNF-α, IL-6 and IL-1β in RAW 264.7 cells. The treatment of six enzymatic hydrolysates of U. major (alcalase, α-chymotrypsin [α-Chy], trypsin, pepsin, neutrase, protamex) significantly increased the production of NO in RAW 264.7 cells, with α-Chy having the greatest effect. This hydrolysate was fractionated by two ultrafiltration membranes at 3 and 10 kDa to created three fractions (below 3 kDa, between 3 and 10 kDa, and above 10 kDa). Of these, the < 3 kDa and >10 kDa fractions showed significant increases in NO production. These two fractions also induced PGE<sub>2</sub> production in RAW 264.7 cells and showed significant increases in the expression of all cytokines studied. These results suggest that enzymatic hydrolysate from U. major is a potentially useful food material with immune-potentiating effects.
혈관내피세포에서 TNF-α로 유도되는 혈관염증에 대한 쏙(Upogebia major) 효소가수분해물의 억제 효과
김소연 ( So-yeon Kim ),양지은 ( Ji-eun Yang ),송재희 ( Jae-hee Song ),맹상현 ( Sang-hyun Maeng ),이지현 ( Ji-hyun Lee ),윤나영 ( Na-young Yoon ) 한국수산과학회(구 한국수산학회) 2018 한국수산과학회지 Vol.51 No.2
Arteriosclerosis is the major cause of coronary artery and cerebrovascular disease, which are leading causes of death. Pro-inflammatory cytokines induce injury to vascular endothelial cells by increasing cell adhesion molecules, leading to vascular inflammation, a major risk factor for the development of arteriosclerosis. In the current study, we investigated the inhibitory effect of enzymatic hydrolysate from Japanese mud shrimp Upogebia major on the inflammation of tumor necrosis factor-α (TNF-α)-stimulated human umbilical vein endothelial cells (HUVECs). We first evaluated the antioxidant and angiotensin I-converting enzyme (ACE) inhibitory activities of eight U. major enzymatic hydrolysates: alcalase, papain, α-chymotrypsin (α-Chy), trypsin, pepsin, neutrase, protamex and flavourzyme. Of these, α-Chy exhibited potent antioxidant and ACE inhibitory activities. The α-Chy hydrolysate was fractionated by two ultrafiltration membranes of 3 and 10 kDa. The α-Chy hydrolysate of U. major and its molecular weight cut-off fractions resulted in a significant reduction in NO production and a decrease in cell adhesion molecules [vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1) and endothelial-selectin (E-selectin)] and pro-inflammatory cytokines [interleukin-6 (IL-6), interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1)] in TNF-α-stimulated HUVECs. These results suggest that enzymatic hydrolysate from U. major can be used in the control and prevention of vascular inflammation and arteriosclerosis.