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미니돼지에서 자가 피부유래 간엽성 줄기세포를 이용한 상악동저 거상술
변준호(June-Ho Byun),강은주(Eun-Ju Kang),맹근호(Geun-Ho Maeng),노규진(Gyu-Jin Rho),강동호(Dong-Ho Kang),이종실(Jong-Sil Lee),박봉욱(Bong-Wook Park) 대한구강악안면외과학회 2010 대한구강악안면외과학회지 Vol.36 No.2
Introduction: In our previous studies, we isolated porcine skin-derived mesenchymal stem cells (pSDMSCs) from the ears of adult miniature pigs and evaluated the pluripotency of these pSDMSCs based on expressions of transcription factors, such as Oct-4, Sox-2, and Nanog. Moreover, the characteristic of mesenchymal stem cells was revealed by the expression of various mesenchymal stem cell markers, including CD29, CD44, CD90, and vimentin. The aim of this study was to evaluate in vivo osteogenesis after maxillary sinus lift procedures with autogenous pSDMSCs and scaffold. Materials and Methods: The autogenous pSDMSCs were isolated from the 4 miniature pigs, and cultured to 3rd passage with same methods of our previous studies. After cell membranes were labeled using a PKH26, 1×107 cells/100 μL of autogenous pSDMSCs were grafted into the maxillary sinus with a demineralized bone matrix (DBM) and fibrin glue scaffold. In the contralateral control side, only a scaffold was grafted, without SDMSCs. After two animals each were euthanized at 2 and 4 weeks after grafting, the in vivo osteogenesis was evaluated with histolomorphometric and osteocalcin immunohistochemical studies. Results: In vivo PKH26 expression was detected in all specimens at 2 and 4 weeks after grafting. Trabecular bone formation and osteocalcin expression were more pronounced around the grafted materials in the autogenous pSDMSCs-grafted group compared to the control group. Newly generated bone was observed growing from the periphery to the center of the grafted material. Conclusion: The results of the present study suggest that autogenous skin-derived mesenchymal stem cells grafting with a DBM and fibrin glue scaffold can be a predictable method in the maxillary sinus floor elevation technique for implant surgery.
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LED 디스플레이 숙련도시험 시료의 안정성 판정 방법 연구
남효재(Hyo-Jae Nam),맹근호(Geun-Ho Maeng),정영호(Yeong-Ho Jeong) 한국조명·전기설비학회 2011 한국조명·전기설비학회 학술대회논문집 Vol.2011 No.11
The confidence of measurement results is important in native and foreign. Although the measurement uncertainty is used for confidence of measurement results, but it is difficult to confirm that there are the errors in the measurement methods and bias in the measurement results continuously. It is difficult to solve these problems, because there is no proficiency testing in LED display field. The coordinator of proficiency testing should consider homogeneity, stability, assigned value, standard deviation and performance statistics to realize the purpose of proficiency testing. The regression analysis is suggested for stability evaluation of artifact in this thesis. The relations between time and measurement results could be identified by regression analysis.
ScienceON
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