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RNA - dependent RNA 중합효소 (重合酵素) Mofif 를 포함하는 오돈토글로썸 윤문 바이러스 52 kD 유전자의 특성과 다른 Tobamovirus 와의 비교
유기현(Ki Hyun Ryu),최장원(Chang Won Choi),김수중(Su Soong Kim),박원목(Won Mok Park) 한국식물학회 1995 Journal of Plant Biology Vol.38 No.2
Complementary DNA of the genomic RNA of odontoglossum ringspot virus Cymbidium strain (ORSV-Cy) was synthesized from polyadenylated viral RNA and cloned. Selected clones containing the viral RNA-dependent RNA polymerase gene of the virus has been sequenced by automated sequencing system. The complete nucleotide sequence of an open reading frame is 1377 base pairs in length, and encodes a protein of 458 amino acids about 52,334 D. The 52 kD protein of ORSV is 16 amino acids shorter than that of tobacco mosaic virus (TMV). The 52 kD protein of ORSV shares four sequence motifs characteristic of viral RNA-dependent RNA polymerase. Comparison of the ORSV 52 kD protein sequence with that of other five viruses in tobamovirus group showed 76.0 to 60.7% homologies at the amino acid level and the conservation of the four motifs between the viruses.