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나노셀룰로오스의 형태적 특징 연구를 위한 투과전자현미경용 최적의 그리드 선택과 처리 방법
권희애(Hee-Ae Kwon),신수정(Soo-Jeong Shin),권오경(Ohkyung Kwon) 한국펄프·종이공학회 2017 펄프.종이기술 Vol.49 No.1
Morphological characteristics of nanocelluloses are necessary to advance our understanding of role within suspension and composite systems. When the shape is investigated by transmission electron microscope, nanocelluloses are required to be separated individual particles with minimum cross-over and overlap. The dispersion of nanocelluloses is significantly affected by surface chemistry of nanocellulose, pH of suspension, surface characteristics of supporting film of TEM grid, and features of stains for negative staining. For nanocelluloses with the hydrophilic surface, supporting film of a TEM grid is needed to be not hydrophilic but also to match pHs of nanocellulose suspension and negative staining solution. This paper provides a brief overview of conditions of an optimal dispersion of nanocelluloses on a TEM grid for quantitative determination of the shape of nanocelluloses.
송우용(Woo-Yong Song),권희애(Hee-Ae Kwon),김병로(Byung-Ro Kim),신수정(Soo-Jeong Shin) 한국펄프·종이공학회 2016 펄프.종이기술 Vol.48 No.4
Hemicelluloses difference between Korean red pine and Geumgang pine was investigated by acid hydrolysis and ¹H-NMR spectroscopic method. In galactoglucomannan of sapwood, acetic acid:galactose:mannose ratio was 4.0:1.9:11.5 for Geumgang red pine but 3.8:6.1:10.2 for Korean red pine, which meant Korean red pine had galactose-rich glucomannan but Geumgang pine had galactose-poor glucomannan. In arabinoglucuronoxylan, arabinose:glucuronic acid:xylose ratio was 0.9:0.9:4.2 in Geumgang red pine but 1.0:1.2:4.0 in Korean red pine, which meant higher arabinose to xylose ratio in Geumgang red pine than Korea red pine. Based on hemicelluloses analysis, it is possible to distinguish between Korean red pine and Geumgang red pine.