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음폐수 처리 실규모 혐기소화조의 박테리아 군집 구조 고찰
이준엽 ( Joonyeob Lee ),황병철 ( Byungchul Hwang ),구태완 ( Taewoan Koo ),황석환 ( Seokhwan Hwang ) 한국폐기물자원순환학회(구 한국폐기물학회) 2014 한국폐기물자원순환학회 심포지움 Vol.2014 No.2
Bacterial communities were investigated using 454 pyrosequencing in two different full-scale anaerobic digesters treating food waste-recycling wastewater (FRW). Seasonal samples were collected for maximum 2 years from a Continuously-stirred tank Thermophilic (CT) digester in Gwangju. Key bacterial genera were determined in terms of operating temperature and reactor configurations from monitoring of temporal variation in full-scale systems for long time period. It was revealed that some process variables (e.g., Residual lipid concentration) had significant influence on variations in bacterial community structure and even in process stability. This result may be valuable reference to select appropriate seed to start up other full-scale reactors treating FRW and valuable reference for other colleague researchers who have plan to study further in optimization and process modeling in anaerobic digestion of FRW.
음폐수 처리 실규모 혐기소화조의 메탄균 군집 구조 고찰
송민준 ( Minjoon Song ),이준엽 ( Joonyeob Lee ),황병철 ( Byungchul Hwang ),구태완 ( Taewoan Koo ),황석환 ( Seokhwan Hwang ) 한국폐기물자원순환학회(구 한국폐기물학회) 2014 한국폐기물자원순환학회 심포지움 Vol.2014 No.2
Structural shift of methanogen communities of a full-scale biogas reactor was examined using 454 pyrosequencing of 16S rRNA gene sequences. Samples were quarterly collected for two years from a continuously-stirred tank digester (CT, location: Gwangju) treating food waste-recycling wastewater operated at thermophilic conditions. The pyrosequencing generated 8 251 sequences representing 2 phyla, 4 classes, 6 orders, 6 families, 10 genera, and 35 species of잸rchaea. The most abundant sequences at the species level originated from Methanothermobacter wolfeii (CT1,CT7 and CT8) and Methanoculleus thermophiles (CT2 to CT6) in the reactor. These highly versatile species represent organotrophs, including mainly hydrogenotrophic methanogens capable of converting hydrogen and carbon dioxide into methane. In this regard, the methane production of this reactor follow the hydrogenotrophic methanogenesis pathway utilizing hydrogen and carbon dioxide as a carbon source. This result might be supplied as a selective reference of relevant works to start-up fullscale digesters (inoculum reference) and enhance process efficiency (optimization).