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Lactobacillus crispatus KLB46의 스트레스 전처리시 열 내성 증진효과
곽대영(Dae-Yung Kwak),강창호(Chang-Ho Kang),전한을(HanEul Jeon),소재성(Jae-Seong So) 한국생물공학회 2014 KSBB Journal Vol.29 No.2
Lactobacilli, the dominant species of microorganisms in the vaginal flora of healthy women, play important roles to prevent bacterial vaginosis and other sexually transmitted diseases. In this study, we carried out studies on stress adaptation prior to various stress treatment. We found that heat or salt adapted KLB46 showed higher cell viability than non adapted upon heat stress at 60℃ for 20 min. When chloramphenicol was added during the adaptation process, heat tolerance was abolished. This result suggested that de novo protein synthesis was essential during adaptation.
유독 이온성 액체의 장기 노출에 대한 Shewanella oneidensis MR-1의 반응
한상현(Sang Hyun Han),강창호(Chang-Ho Kang),곽대영(Dae Yung Kwak),하성호(Sung Ho Ha),구윤모(Yoon-Mo Koo),소재성(Jae-Seong So) 한국생물공학회 2013 KSBB Journal Vol.28 No.2
In this study, we investigated the effects of longterm exposure to ionic liquid (IL) on Shewanella oneidensis MR-1 (MR-1). MR1 was acclimated through repeated exposure to IL. The acclimated strain was named as S. oneidensis SH-1 (SH-1) and compared with MR-1 in various aspects including morphology, cell surface hydrophobicity (CSH), motility, and fatty acid. Compared to the MR-1, SH-1 showed elongated cell shape on scanning electron microscopy. Upon exposure to IL, hydrophobicity of SH-1 (28.2%) was higher that of MR-1 (3.3%). In contrast, motility of SH-1 (7 mm) was lower than that also of MR-1 (22 mm), and branched chain fatty acid of SH-1 was lower than that of MR-1, 27.6% and 41.1%, respectively.
강창호(Chang-Ho Kang),정호건(Ho Geon Jeong),구자륭(Ja-Ryong Koo),전은진(Eun Jin Jeon),곽대영(Dae Yung Kwak),홍채환(Chae Hwan Hong),김시환(Si Hwan Kim),서지연(Ji Yeon Seo),한도석(Do Suck Han),소재성(Jae-Seong So) 한국생물공학회 2012 KSBB Journal Vol.27 No.3
Lactic acid is an important product arising from the anaerobic fermentation by lactic acid bacteria (LAB). It is used in the pharmaceutical, cosmetic, chemical, and food industries as well as for biodegradable polymer and green solvent production. The poly lactic acid (PLA) is an important material for bio-plastic manufacturing process. For PLA production by new LAB, we screened LAB isolates from shellfish. A total of 28 LAB were isolated from various shellfishes. They were all Gram positive, oxidase and catalase negative. Based on API 50CHL kit, 7 strains among the 28 isolates were identified as Lactobacillus plantarum, 6 strains as Lactobacillus delbrueckii, 5 strains as Leuconostoc mesenteroides, 3 strains as Lactobacillus brevis, 2 strains as Lactococcus lactis, 1 strain as Lactobacillus salivarius, 1 strain as Lactobacillus paracasei, 1 strain as Lactobacillus pentosus, 1 strain as Lactobacillus fermentum and 1 strain as Pediococcus pentosaceu. Also, we examined the amount of total lactic acid produced by these new strains by HPLC analysis with Chiralpak MA column. One strain E-3 from Mytilus edulis was indentified as Lactobacillus plantarum and found to produce 20.0 g/L of D-form lactic acid from 20 g/L of dextrose. Further studies are underway to increase the D-lactic acid production by E-3.