조직배양에 의한 반하〔Pinellia ternata(Thunb.) Briet〕의 대량번식에 관한 연구

崔定植,羅義植 韓國作物學會 1986 Korean journal of crop science Vol.31 No.1

반하〔Pinellia ternata(Thunb.) Briet〕의 조직을 기내배양하여 callus 유기, 기관분화 및 자구를 증식시키는데 적합한 배지와 배양부위를 선정코저 Mur-ushige & Skoog 배지에 2,4-D와 kinetin의 첨가량을 달리하여 검토하였고 또한 배양온도 및 배지의 pH 영향을 조사하였던 바 그 결과를 요약하면 다음과 같다. 1. Callus 유기는 배양 5~7일 후에 엽조직 및생장점조직의 절단면에서 관찰할 수 있었으며 2,4-D 2.0mg/ +kinetin 0.2mg/ 을 첨가한 구에서 callus의 형성 및 생장이 가장 양호하였다. 2. 자구의 형성은 엽조직 및 생장점조직을 2,4 -D 2.0mg/ +kinetin 0.2mg/ 또는 2.4-D 2.0g/ +kinetin 2.0mg/ 을 첨가한 배지에서 양호하였고 근 및 괴경조직에서는 자구가 전혀 ?성되지 않았다. 3. 엽의 부위별 자구형성능력은 Petiole이 자구형성수, 크기, 엽면적 및 생체중에 있어서 marginal meristem, minor vein area 및 intercostal area 보다 양호하였다. 4. 경의 부위별 자구형성능력은 상부위 조직일수록 자구의 형성수 크기, 엽면적 및 생체중이 양호하였으나 하부위일수록 불량하였다. 5. 배양온도는 25℃ 에서 callus 유기, 크기 및 생체중이 가장 양호하였다. 6. 배지의 pH는 4.5~7.5에서 모두 callus가 유기되었으나 그중 pH 6.0 배지에서 가장 양호하였다. 7. 재생되는 식물체 일부가 광엽화되는 경향이었으며 소수의 albino 식물체와 변이체도 관찰할 수 있었다. In order to find out the best media, explants and environmental conditions for induction of calluses and organogeneses of Pinellia ternata (Thunb.) Breit in vitro, various parts of adult have been cultured on Murashige & Skoog's medium containing various levels of 2,4-dichlorophenoxy acetic acid(2,4-D) and kinetin. The results obtained were as follows: Calluses were induced from the surface of apical meristem and leaf tissue. Formation and growth of calluses in petiole ex plants were best on the MS medium complemented with 2,4-D 2.0 mg/l and kinetin 0.2mg/l. But callus formation in stem ex plants of the nearest tuber was not induced at all kinds of media. Plantlets occured at all treatment except absence of growth regulator. Their numbers, size, leaf and fresh weight were promoted by 2,4-D 2.0mg/l and kinetin 0.2mg/l. Root growth was increased on the medium containing higher 2,4-D concentrations. Size and fresh weight of callus were increased at 25~circC compared with 10, 20 and 30~circC , respectively. Optimal pH value was at 6.0 for growth of callus. Morphological aberrations were observed in plantlets, especially in regenerated leaves. The separation of the broad leaved plantlets and albino were observed in some cultures. Growth of plantlets after transplantation was best in pots with the sterilized vermiculte. But abnormal variants withered up.

HAI 제어기에 의한 IPMSM 드라이브의 최대토크 제어

최정식,남수명,정동화 국립7개대학공동논문집간행위원회 2005 공업기술연구 Vol.5 No.-

Interior permanent magnet synchronous motor(IPMSM) has become a popular choice in electric vehicle applications, due to their excellent power to weight ratio. The paper proposed maximum torque control of IPMSM drive using hybrid artificial intelligent(HAI) controller. The control method is applicable over the entire speed range and considered the limits of the inverter's current and voltage rated value. Also, this paper proposed speed control of IPMSM using adaptive learning mechanism fuzzy neural network(ALM_FNN) and estimation of speed using artificial neural network(ANN) controller. The proposed control algorithm is applied to IPMSM dreve system controlled ALM-FNN and ANN controller, the operating characteristics controlled by maximum torque control are examined in detail. Also, this paper proposed the analysis results to verify the effectiveness of the ALM-FNN and ANN controller.

선내 제조된 혼합연료 품질 개선을 위한 초음파 적용 가능성에 관한 연구

최정식,주해지,한원희 해양환경안전학회 2017 해양환경안전학회지 Vol.23 No.1

이 연구는 초음파 에너지의 공동현상(Cavitation)을 이용하여 선내 제조된 혼합연료유의 문제점을 개선하고 혼합연료유의 안정적인 사용이 가능하도록 하여 선박운용비의 상당부분을 차지하는 연료비를 절감하고자 한다. 실험은 선내 혼합연료유 제조 방식을 모사하여 선박용 연료유 M.G.O(Marine Gas Oil)와 MF-180(Marine Fuel-oil 180)를 각각 부피비 기준으로 0.25:0.75 및 0.75:0.25 비율로 혼합하였으며 초음파 처리장치를 이용하여 혼합연료유에 초음파 에너지를 직접 조사하여 초음파 에너지가 혼합연료유에 미치는 영향에 관해 고찰하였다. 실험결과, 선내 혼합유 제조시 보고되었던 문제점을 확인하였으며, 혼합시료유의 초음파 조사 후 잔류탄소량은 최대 28.4 % 감소하였다. 또한, 잔류탄소량 감소 및 분산 안정성 분석결과를 토대로 초음파 에너지에 의한 캐비티의 붕괴압이 연료입자 미립화에 효과가 있고, 중질연료유가 많이 함유된 혼합연료유의 일시적인 가용성을 높일 수 있을 것으로 판단되었다. In this study, contributes to improving the state of this problem using cavitation by ultrasonic energy to reduce fuel costs, which take up a considerable part of ship operation costs, by making the use of on-board blended fuel oil more stable. An experiment simulating on-board blending methods was completed. Fuel (M.G.O & MF-180) was mixed at a volume ratio of 0.25:0.75 and, 0.75:0.25, and the effect of ultrasonic energy on blended fuel oil was examined after applying ultrasonic energy to blended fuel oil using an ultrasonic treatment unit. With the results, we confirmed the blending problem reported by vessels and residual carbon was reduced by up to 28.4%. In addition, based on the results for reduction of residual carbon content and dispersion stability, it was confirmed that the collapse pressure of the cavity due to the ultrasonic energy was effective to atomization of fuel particle and the temporary availability of mixed fuel containing a heavy fuel increased.

해외연수기 : 공무원의 채취가 잘 스며든 복지의 낙원 스위스

최정식 대한지방행정공제회 2000 地方行政 Vol.49 No.560

초음파 에너지를 활용한 선박 연료유 개질에 관한 기초연구

최정식,한원희 해양환경안전학회 2019 해양환경안전학회 학술발표대회 논문집 Vol.2019 No.04

[LAW & LIFE] 공동주택의 층간 소음분쟁

최정식 전력문화사 2015 Electric Power Vol.9 No.10

水稻 機械移秧 栽培技術에 관한 硏究

崔定植,盧承杓 전북대학교 농업과학기술연구소 1979 農大論文集 Vol.10 No.-

Since unemploied or agricultural labours are bfing abforbed in thf fast-flowing second andthird industries, the short of farm labours befumes infvitable and thereforf, labour-savingculture by mfchanization of agriculture is urgently needed.The prrsent expfriment was cfrried out Ivith thE c3.n'linatio.1 of diffcrfnt seeding rates,fertilizering leveis and tFansplanting dltji for Milfan3 23 t) invBstigBte thf limiting periodfor safe transplantinf rfiat?d Ivith labo·Ar-saving culture of ric2 plants.The resuits are as follolvs.1) Juvenile seedlingf shrued n3 grflt differfnff3 in sBEBlinT chBra2t2ristics however, thegrowlh o: seedlingf Ivas Ingre favorfd as tIIB an]lIt o: all:ift ff?:ilifB,'s inci·fased.2) Seeding rates are favrured about 159∼1839/0. 18%2 for thf raisinf of j·fvenile seedlings,,Ibout 120~1509/0. 18u3 for mrdium seedlings and a)1311 8)~110f/3. 18 ul foF mltdrf .3fedlings.3) Early transplantinT teHdfd to shlu le33 An'4,;3inf plaltE or dfad flaltl and bftter rootingparticularly in the case of juvenile seedlings.4) Alfo, early transplanting showed high pfrcfntagfs of ripfned grains as well as lolvpercentages of effective tiller3 bffaufe of 481aye0 tillering.5) As we recognize that the 20th of August is the limiting heading date for rice plants inJeonbug area, we may sugeest May 23 a3 limiting tran3planting dBt2 for jfvfnile seedlings June10 for mature-seedling3. In case of thf wEBthfr c31fi1io,Is likf thr year of 1977 transplantingof juvenile seedlings is p93sible until Jun2 10 with some risks.

摘葉處理가 大豆의 收量 및 諸 形質에 미치는 影響

崔定植,金永斗 전북대학교 농업과학기술연구소 1983 農大論文集 Vol.14 No.-

The influence of 4 levels of defoliation(0, 1/3, 2/3 and 3/3) on seed yield and other important agronomic characteristics were evaluated at each of 5 stages of growth(R_1, R_2, R_3, R_4, R_5, R_6). The indeterminate cultivar 'Clark' was engaged in this experiment and the experiment design was a split plot with 5 stages of soybean growth as whole plots and 4 levels of defoliation as subplots, with 3 replicates. The results were summarized as follows : 1. Development of new leaf are after defoliation was greater at the initial stages than at the later stages. 2. An increase in defoliation at growth stage R_2 was delayed in maturity, but hastened at R_6. 3. The more levels of defoliation increased and stages of defoliation were early, the more stem height and stem weight reduced. Main stem nodes and number of branches did not significantly reduce at any stages of defoliation, but any level of defoliation did. 4. Number of pods and number of speeds per plant reduced linearly with levels of defoliation and stages ; maximum number of pods and seeds per plant reduction of 55.4 and 58.5 occurred in 3/3 defoliation at stages R_4 and R_5, respectively. 100 seed weight did not significantly reduce at stages of defoliations, but any level of defoliation did. 5. Seed yield reduced severely with levels of defoliation and stages ; maximum seed yield reduction of 31.8 kg/10a(85.6%) occurred in 3/3 level of defoliation at stage R_5. 6. Levels of defoliation had significant negative correlation with number of pods, number of seeds per plant and seed yield at all stage, but maturity, stem height and seed weight were different at stages of defoliation. 7. The mean oil content was 19.3, 17.8 and 16.8% respectively, in 1/3, 2/3 and 3/3 level of defoliation, but protein content had no significant effect at defoliation. 8. Germination percentage was 86.2 at 3/3 level of defoliation compared with undefoliation.

실습선을 이용한 주 추진기관의 배기배출물의 실시간 계측

최정식,이상득,이경우,천강우,남연우,윤석훈,최재혁,Choi, Jung-Sik,Lee, Sang-Deuk,Lee, Kyoung-Woo,Chun, Kang-Woo,Nam, Youn-Woo,Yoon, Seok-Hun,Choi, Jae-Hyuk 해양환경안전학회 2013 海洋環境安全學會誌 Vol.19 No.5

본 연구에서는 한국해양대학교 실습선 한바다를 이용하여 선박의 입 출항 및 약 150 rpm의 정속 운항 할 때 주기관에서 배출되는 배기가스를 실시간 계측하였다. 실측 결과 질소산화물의 농도는 정속 운항시 800 ppm 에서 1,000 ppm 사이인데 반해, 입 출항시에는 210 ppm 에서 1,230 ppm 까지 큰 범위에서 값이 변화하였다. 일산화탄소의 농도 역시 정속운항 상태 보다 입 출항시 측정값의 변화가 크게 나타났다. 이러한 결과는 입 출항시 가능한 한 주기관의 부하변동이 급격히 발생되지 않도록 하는 선박 조종 스킬이 필요하다는 것을 의미한다. 또한, 배기가스 저감 기술의 적용에 있어 입 출항시와 정속 운항할 때 그 차이를 고려할 필요가 있다는 것을 나타낸다. In this study, we conducted real-time measurement at the ship arrivals and departures at the port and at a constant speed of 150 rpm for exhaust emissions from a main engine installed on the training ship, HANBADA, of Korea Maritime University. The result showed that the concentration of nitrogen oxide was measured in the range of 800 ppm to 1,000 ppm at constant speed mode. On the other hand, the concentration of nitrogen oxide during ship arrivals and departures was significantly fluctuated between 210 ppm and 1,230 ppm. And, the concentration of carbon oxide at the arrivals and departures was also larger than that of at constant speed mode. These results show that the ship maneuvering skills to prevent a sudden load change of main engine at the arrivals and departures of ship is needed. Additionally, it means that the difference of exhaust emissions generated between the constant speed mode and the arrival/departure has to be considered when invented many technologies are adopted into the reduction technologies of air pollutants from ships.

湖南米의 品質向上을 爲限 調査 硏究(II)

崔定植 전북대학교 농업과학기술연구소 1976 農大論文集 Vol.7 No.-

In order to investigte the difference between Honam and Gyeonggi rices in the content of amylose and protein to concern their Quality, five places were established each in Gyeonggi and Honamareas for sampling and rices which were marketing and carring were collected from November in 1974 to April in 1975 at intervals of a month. The results obtained are as follows : 1. In the content of amylose and protein, there was no difference between Honam and Gyeonggi rice. 2. Possibly there was distinguished difference in the content of amylose and protein according to sampling dates. 3. In rices of Honam and Gyeonggi areas, the later the sampling date is, the more amylose content is, on the other hand, the less protein content is.