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        Field monitoring of splitting failure for surrounding rock masses and applications of energy dissipation model

        Wang, Zhi-shen,Li, Yong,Zhu, Wei-shen,Xue, Yi-guo,Jiang, Bei,Sun, Yan-bo Techno-Press 2017 Geomechanics & engineering Vol.12 No.4

        Due to high in-situ stress and brittleness of rock mass, the surrounding rock masses of underground caverns are prone to appear splitting failure. In this paper, a kind of loading-unloading variable elastic modulus model has been initially proposed and developed based on energy dissipation principle, and the stress state of elements has been determined by a splitting failure criterion. Then the underground caverns of Dagangshan hydropower station is analyzed using the above model. For comparing with the monitoring results, the entire process of rock splitting failure has been achieved through monitoring the splitting failure on side walls of large-scale caverns in Dagangshan via borehole TV, micro-meter and deformation resistivity instrument. It shows that the maximum depth of splitting area in the downstream sidewall of the main power house is approximately 14 m, which is close to the numerical results, about 12.5 m based on the energy dissipation model. As monitoring result, the calculation indicates that the key point displacement of caverns decreases firstly with the distance from main powerhouse downstream side wall rising, and then increases, because this area gets close to the side wall of main transformer house and another smaller splitting zone formed here. Therefore it is concluded that the energy dissipation model can preferably present deformation and fracture zones in engineering, and be very useful for similar projects.

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        Genetic Organization of ascB-dapE Internalin Cluster Serves as a Potential Marker for Listeria monocytogenes Sublineages IIA, IIB, and IIC

        ( Jian Shun Chen ),( Chun Fang ),( Ning Yu Zhu ),( Yong Hui Lv ),( Chang Yong Cheng ),( Yi Jiang Bei ),( Tianlun Zheng ),( Wei Huan Fang ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.5

        Listeria monocytogenes is an important foodborne pathogen that comprises four genetic lineages: I, II, III, and IV. Of these, lineage II is frequently recovered from foods and environments and responsible for the increasing incidence of human listeriosis. In this study, the phylogenetic structure of lineage II was determined through sequencing analysis of the ascB-dapE internalin cluster. Fifteen sequence types proposed by multilocus sequence typing based on nine housekeeping genes were grouped into three distinct sublineages, IIA, IIB, and IIC. Organization of the ascBdapE internalin cluster could serve as a molecular marker for these sublineages, with inlGHE, inlGC2DE, and inlC2DE for IIA, IIB, and IIC, respectively. These sublineages displayed specific genetic and phenotypic characteristics. IIA and IIC showed a higher frequency of recombination (ρ/θ). However, recombination events had greater effect (r/m) on IIB, leading to its high nucleotide diversity. Moreover, IIA and IIB harbored a wider range of internalin and stress-response genes, and possessed higher nisin tolerance, whereas IIC contained the largest portion of low-virulent strains owing to premature stop codons in inlA. The results of this study indicate that IIA, IIB, and IIC might occupy different ecological niches, and IIB might have a better adaptation to a broad range of environmental niches.

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