Poster Session : The Effects of A Long Term Creatine Supplementation on the Kidney Function in Elite Weight Lifters

( Zhou Xiang Shan ),( Hyo Jak Ahn ),( Dong Cheol Yeom ),( Chang Keun Kim ) 한국체육학회 2013 In Pursuit of Evidence-Based Sport and Exercise Sc Vol.0 No.-

Immunotherapeutic Effects of Dendritic Cells Pulsed with a Coden-optimized HPV 16 E6 and E7 Fusion Gene in Vivo and in Vitro

Zhou, Zhi-Xiang,Li, Dan,Guan, Shan-Shan,Zhao, Chen,Li, Ze-Lin,Zeng, Yi Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9

Background: Cervical cancer is the second most common cause of cancer related death of women. Persistent HPV infection, especially with high-risk types such as HPV16 and HPV18, has been identified to be the primary cause of cervical cancer. E6 and E7 are the major oncoproteins of high-risk HPVs, which are expressed exclusively in HPV infected tissues, and thereby represent ideal therapeutic targets for immunotherapy of cervical cancer. Materials and Methods: In this work, we used recombinant adenovirus expressing coden-optimized HPV16 E6 and E7 fusion protein (Ad-ofE6E7) to prime dendritic cells (DC-ofE6E7), to investigate the ability of primed DC vaccine in eliciting antitumor immunity in vitro and vivo. Results: Our results indicated that DC-ofE6E7 vaccine co-culturing with splenocytes could strongly induce a tumor-specific cytotoxic T lymphocyte (CTL) response and kill the TC-1 cells effectively in vitro. Moreover, DC-ofE6E7 vaccine induced protective immunity against the challenge of TC-1 cancer cells in vivo. Conclusions: The results suggested that the HPV16 ofE6E7 primed DC vaccine has potential application for cervical cancer immunotherapy.

장기간의 크레아틴 섭취가 역도선수 신장 기능에 미치는 영향

선주상 ( Zhou Xiang Shan ),안효작 ( Hyo Jak Ahn ),염동철 ( Dong Cheol Yeom ),김창근 ( Chang Keun Kim ) 한국체육대학교 체육과학연구소 2013 스포츠사이언스 Vol.31 No.1

이 연구의 목적은 장기간 크레아틴 섭취가 역도선수의 신장기능에 미치는 영향을 알아보는데 있다. 연구 대상자는 평소 꾸준히 크레아틴을 섭취해 온 역도 국가대표선수 남녀 57명으로, 집단은 크레아틴 섭취 기간에 따라 1년 미만(n=10), 1년-3년 (n=15), 3년-5년(n=16), 5년 이상(n=16)으로 구분하였다. 신장기능을 평가하기 위해 혈청과 뇨에서 관련 변인(크레아틴, 크레아티닌, 뇨산, 시스타틴-C)을 분석하였다. 실험결과, 혈청 및 뇨에서 크레아틴, 크레아티닌, 뇨산 그리고 시스타틴-C는 모두 집단 간에 유의한 차이를 나타내지 않았다. 또한, 측정된 변인들은 혈액이나 뇨에서 모두 정상 농도범위를 유지했다. 그러나 뇨에서 크레아티닌과 뇨산은 정상 농도범위 보다 약간 높게 나타났으며, 혈청에서 사구체여과율은 정상범위 보다 약간 낮게 나타났다. 요약하면, 혈청과 뇨에서 분석한 변인들은 1년 미만에서 5년 이상의 크레아틴 섭취기간에 따른 집단 간에 차이를 보이지 않았다. 그러나 모든 집단에서 뇨 크레아티닌과 뇨산 수준은 정상수준보다 높았고, 사구체여과율은 더 낮은 수준을 보였다. 그러므로, 이러한 결과들을 확인하기 위한 후속연구가 필요할 것으로 사료된다. The aim of the present study was to investigate the effect of a long term creatine supplementation on renal function in weight lifters. Fifty seven weight lifters who have been supplemented creatine was participated in the experiment, and divided into four groups of under 1 yr (n=10), 1 to 3 yrs (n=15), 3 to 5 yrs (n=16), and over 5 yrs (n=16) by the period of creatine intake. Serum and urine creatine, creatinine, uric acid, and cystatin-C were analysed to evaluate renal function. There was no significant differences among the groups, not only by the measurement of serum creatine, creatinine, uric acid, cystatin- C, but also in urine as well. All measured valuables to evaluate renal function maintained normal concentration either in blood or in urine. But urine creatinine and uric acid level were maintained higher than normal concentration, and GFR level in serum was lower than normal level in all groups. In summary, there were no difference among the different durations of creatine supplementation of under one year to more than five years in the parameters measured in serum and urine. However urine creatinine and uric acid level were maintained slightly higher, and GFR level in serum was slightly lower in all groups. Therefore, it is highly recommended further research to confirm these findings in the future.

Meta-analysis of Gene Expression Data Identifies Causal Genes for Prostate Cancer

Wang, Xiang-Yang,Hao, Jian-Wei,Zhou, Rui-Jin,Zhang, Xiang-Sheng,Yan, Tian-Zhong,Ding, De-Gang,Shan, Lei Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1

Prostate cancer is a leading cause of death in male populations across the globe. With the advent of gene expression arrays, many microarray studies have been conducted in prostate cancer, but the results have varied across different studies. To better understand the genetic and biologic mechanisms of prostate cancer, we conducted a meta-analysis of two studies on prostate cancer. Eight key genes were identified to be differentially expressed with progression. After gene co-expression analysis based on data from the GEO database, we obtained a co-expressed gene list which included 725 genes. Gene Ontology analysis revealed that these genes are involved in actin filament-based processes, locomotion and cell morphogenesis. Further analysis of the gene list should provide important clues for developing new prognostic markers and therapeutic targets.

Application of Highly Charged PAC and Polyamine admixture in Neutral Papermaking

YANYONG-XIANG,CHEN FU-SHAN,WANGLI-JUN,ZHOU LIN-JIE 한국펄프·종이공학회 2006 한국펄프종이학회 기타 간행물 Vol.- No.-

Studied that the relationship between the charge density of PAC and its alkalinity, as well as the influences of PAC in paper sizing. Study results showed that PAC with higher charge density brought higher sizing degree when the same amount of Al₂O₃ content was dosed. However, there was no direct relationship between PAC charge density and its alkalinity, and higher PAC alkalinity can’t improve its charge density. It was also found that the admixture (PPAC) of polyamine and PAC had higher charge density than PAC. The effects of PPAC on DRS and RAKD sizing were better than PAC sizing, when dosage and other sizing condition were the same.

思春期 綜合微에 대한 인식

賈春華,周春祥,王天山,崔 勳 대한동의생리학회,대한동의병리학회 2002 동의생리병리학회지 Vol.16 No.5

Nae-Kyung says the puberty is corresponded to the age of 16∼24(male) and 14∼21(female). And that time they are promoted the growth. However, Kidney qi (賢氣) doesn't make average situation during that time. So, the function of five viscera and six entrails fails to be perfect and symptoms appear. I came to the conclusion through the survey of about 200 high school students(male and female) and the theory of oriental medicine the distinctive features (like migraine, amnesia, depression, dysphoria, inappetence, irregular menstruation) came out that time. I defined this distinctive symptoms in boys and girls at puberty 'synthetic symptoms of puberty'. For the medical cure and prevention I prescribed 'Sachun-Nyung' and that has medical benefits for 'Replenishing qi and relieving the spleen(益氣養陰), Soothing the liver and promoting blood circulation(疏肝活血)'

Rational Introduction of Disulfide Bond to Enhance Optimal Temperature of Lipomyces starkeyi α-Dextranase Expressed in Pichia pastoris

Lin Chen,Chao Yu,Xiang Shan Zhou,Yuan Xing Zhang 한국미생물 · 생명공학회 2009 Journal of microbiology and biotechnology Vol.19 No.12

A novel human KRAB-related zinc finger gene ZNF425 inhibits mitogen-activated protein kinase signaling pathway

( Yue Qun Wang ),( Xiang Li Ye ),( Jun Mei Zhou ),( Yong Qi Wan ),( Hua Ping Xie ),( Yun Deng ),( Yan Yan ),( Yong Qing Li ),( Xiong Wei Fan ),( Wu Zhou Yuan ),( Xiao Yang Mo ),( Xiu Shan Wu ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.1

Zinc finger (ZNF) proteins play a critical role in cell growth, proliferation, apoptosis, and intracellular signal transduction. In this paper, we cloned and characterized a novel human KRAB-related zinc finger gene, ZNF425, which encodes a protein of 752 amino acids. ZNF425 is strongly expressed in the three month old human embryos and then is almost undetectable in six month old embryos and in adult tissues. An EGFP-ZNF425 fusion protein can be found in both the nucleus and the cytoplasm. ZNF425 appears to act as a transcription repressor. Over-expression of ZNF425 inhibits the transcriptional activities of SRE, AP-1, and SRF. Deletion analysis indicates that the C2H2 domain is the main region responsible for the repression. Our results suggest that the ZNF425 gene is a new transcriptional inhibitor that functions in the MAPK signaling pathway. [BMB reports 2011; 44(1): 58-63]

저산소 환경과 스포츠 경기력 그리고 대사성 질환

권혁용 ( He Long Quan ),선주상 ( Zhou Xiang Shan ),김영선 ( Young Seon Kim ),최재근 ( Jae Keun Choi ),김창근 ( Chang Keun Kim ) 한국체육대학교 체육과학연구소 2013 스포츠사이언스 Vol.31 No.1

저산소환경이란 대기압의 감소와 산소분압이 감소되는 현상으로 흡기 시 산소분압이 150 mmHg 이하인 조건을 의미한다. 고지환경, 즉 저산소 환경에 대한 연구는 생명과학분야에서 다양하게 중요한 주제로 연구되어 오고 있다. 특히, 스포츠분야에서는 1968년 멕시코 올림픽을 계기로 고지환경이 스포츠 경기력에 지대한 영향을 미친다는 사실과 저산소 환경에서의 적응은 일부 경기종목에 따라서는 저산소 환경에 대한 인체의 적응이 곧 경기력 향상으로 이어질 수 있는 가능성을 입증하기 위한 연구가 많이 진행되어 왔다. 해수면 환경에서 훈련하던 선수들이 갑자기 고지에서 훈련하게 되면 최대능력을 발휘하지 못할 뿐만 아니라 신체의 기능이 고지환경에 적응하기까지 최소한 1주 정도 소요된다. 최근 실내의 산소농도를 조절할 수 있는 기술을 이용하여 휴식 또는 수면시간을 해수면 수준 정상기압의 저산소환경에 노출시켜 고지훈련의 효과를 얻으려는 노력이 시도되고 있다. 고지환경에서 생활하는 동안 인체의 대사 작용이 해수면에서와의 다른 패턴이 보고되면서 부터 스포츠분야 뿐만 아니라 대사성 질환을 연구하는 분야에서도 활발히 연구가 진행되고 있다. 운동의 강도에 따라 달라질 수 있지만 운동강도가 증가 될 수 록 근육 글리코겐 농도의 감소는 비례적으로 증가되며 결과적으로 인슐린 민감도를 증가시킬 수 있다. 고 강도 간헐적운동이 당대사나 당뇨개선에 효과가 있었으나 간헐적 저 강도 운동도 저산소 환경에서 운동했을 때가 정상적인 산소농도에서 운동했을 때 보다 더 효과적이라는 연구 보고가 있다. 즉, 지속적인 운동훈련이 고강도 간헐적 운동 훈련보다 당대사 내지는 당뇨개선에 효과적인 대처방법이 될 수 있다. 저산소환경과 운동은 당대사와 지방산대사의 산화경로에 중대한 영향을 미친다. 운동과저산소 환경은 고지에서 불수위적으로 체중을 감소시키게 될 가능성이 있으나 고지환경에서의 생활이 체중감소에 미치는 영향은 아직 잘 밝혀져 있지않다. 따라서 고지환경이 체중감량에 미치는 메카니즘을 구명하는 것은 비만을 해결하는 중요한 처방으로 이용될 수도 있다. 일반적으로 고지환경이 비만인들의 체중감소를 초래할 수 있는 가능성은 기초대사량의 변화, 추위나 탈진 등과 관련시켜 고려될수 있다. 특히, 고지에서는 고산병으로 인한 식욕감퇴와 에너지섭취량이 감소가 주요 요인으로 고려되고 있다. 이 논문에서는 최근 저산소 환경과 관련되어 논의가 활발하게 이루어지고 있는 저산소 환경이 스포츠 경기력 향상에 미치는 영향뿐만 아니라 저산소 환경이 신체조성을 포함한 대사성 질환과 관련된 변인들을 선행연구 문헌을 통해 고찰하고자 한다. Hypoxic environment can be defined as a complex phenomenon with a decreased partial pressure of oxygenunder the 150mmHg during inhalation by a decreased atmospheric pressure. A various investigations which is related to a decrease oxygen tension or altitude environment has been performed in the field of life science. It has been intensively studied the relationship between hypoxia and exercise performance to prepare for Mexico Olympic Games, and ahs been performed many studies about the adaptation to hypoxia to prove the possibility of improvement of exercise performance. In recent years athletes who has been training from young age at a mild altitude (2000-3000m a.s.l.) has been leading international competitions of middle and long distance run, including marathon run. However, there has been debated whether altitude environment can modulate bodily function, especially exercise performance. Up to today various attempts has been considered to acclimatize to hypoxia efficiently, because athletes may not perform their maximal capacity during early stage of altitude adaptation, and thus recently new technique has developed to control oxygen content at sea level, in terms of normobaric hypoxia. Hypoxic facility can allow the athletes living in normobaric hypoxia and training in normobaric normoxic environment. Various investigations has been performed not only regarding on exercise performance but also on metabolic syndromes according to different response body function to hypoxia compared to normoxia, especially hypoxia has an important role in glucose and fat metabolism. Hypoxia and exercise induce to increase insulin sensitivity and epinephrine to decrease in muscle glycogen, and also does an increase in the expression of hypoxia-inducible factor-1a and leptin concentration to reduce body weight in voluntarily. Therefore, it is important to unravel the mechanism of body weight reduction by the exposure in hypoxic environment and thus may be suggested as a possible countermeasure to the obesity epidemics. Therefore, hypoxia and its related recent studies of exercise performance and metabolic syndromes, including obesity, glucose and fat metabolism will be included in this review.

UBE2W Interacts with FANCL and Regulates the Monoubiquitination of Fanconi Anemia Protein FANCD2

Yingying Zhang,Peitang Huang,Xiaowei Zhou,Lixia Zhao,Chao Li,Hengqi Zhu,Long Xu,Liran Shan,Xiang Liao,Zekun Guo 한국분자세포생물학회 2011 Molecules and cells Vol.31 No.2

Fanconi anemia (FA) is a rare cancer-predisposing ge-netic disease mostly caused by improper regulation of the monoubiquitination of Fanconi anemia complementation group D2 (FANCD2). Genetic studies have indicated that ubiquitin conjugating enzyme UBE2T and HHR6 could regulate FANCD2 monoubiquitination through distinct mechanisms. However, the exact regulation mechanisms of FANCD2 monoubiquitination in response to different DNA damages remain unclear. Here we report that UBE2W, a new ubiquitin conjugating enzyme, could regulate FANCD2 monoubiquitination by mechanisms different from UBE2T or HHR6. Indeed, UBE2W exhibits ubiquitin conjugating enzyme activity and catalyzes the monoubiquitination of PHD domain of Fanconi anemia complementation group L (FANCL) in vitro. UBE2W binds to FANCL, and the PHD domain is both necessary and sufficient for this interaction in mammalian cells. In addition, over-expression of UBE2W in cells promotes the monoubiquitination of FANCD2 and down-regulated UBE2W markedly reduces the UV irradia-tion-induced but not MMC-induced FANCD2 monoubiquiti-nation. These results indicate that UBE2W regulates FANCD2 monoubiquitination by mechanisms different from UBE2T and HRR6. It may provide an additional regulatory step in the activation of the FA pathway.