http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : ( Ruihua Guo ) (검색결과 5 건)
- 무료
- 기관 내 무료
- 유료
-
Assessment of the Cytotoxic and Apoptotic Effects of Chaetominine in a Human Leukemia Cell Line
Jing-Yun Yao,Ruihua Jiao,Changqing Liu,Yupeng Zhang,Wan-Guo Yu,Yan-Hua Lu,Renxiang Tan 한국응용약물학회 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.2
Chaetominine is a quinazoline alkaloid originating from the endophytic fungus Aspergillus fumigatus CY018. In this study, we showed evidence that chaetominine has cytotoxic and apoptotic effects on human leukemia K562 cells and investigated the pathway involved in chaetominine-induced apoptosis in detail. Chaetominine inhibited K562 cell growth, with an IC50 value of 35 nM, but showed little inhibitory effect on the growth of human peripheral blood mononuclear cells. The high apoptosis rates, morphological apoptotic features, and DNA fragmentation caused by chaetominine indicated that the cytotoxicity was partially caused by its pro-apoptotic effect. Under chaetominine treatment, the Bax/Bcl-2 ratio was upregulated (from 0.3 to 8), which was followed by a decrease in mitochondrial membrane potential, release of cytochrome c from mitochondria into the cytosol, and stimulation of Apaf-1. Furthermore, activation of caspase-9 and caspase-3, which are the main executers of the apoptotic process, was observed. These results demonstrated that chaetominine induced cell apoptosis via the mitochondrial pathway. Chaetominine inhibited K562 cell growth and induced apoptotic cell death through the intrinsic pathway, which suggests that chaetominine might be a promising therapeutic for leukemia.
-
( Wu Qiao ),( Tinghan Jia ),( Hongjian Gu ),( Ruihua Guo ),( Ken Kaku ),( Wenhui Wu ) 대한피부과학회 2019 Annals of Dermatology Vol.31 No.6
Background: Vernix caseosa (VC), which is known as a unique human substance, is a biofilm that covers the skin of most human newborns. VC has many biological functions including anti-infective, skin cleansing and skin barrier repair. Objective: In the study, we purpose to investigate the novel effect of lipids extracted from VC on the regulation of filaggrin (FLG) expression and anti-inflammation in normal human epidermal keratinocyte (NHEK) cells. Methods: The lipids were extracted by chloroform/methanol (Folch method) and the major properties of fatty acid methyl esters were determined with gas chromatography-mass spectrometer. The relative viability of NHEK cells was evaluated by Cell Counting Kit 8 assay. The related expression of skin barrier protein was accessed with real-time quantitative polymerase chain reaction, Western blot and Immunofluorescence in NHEK cells with or without poly (I:C). Meanwhile, the changes of thymic stromal lymphopoietin (TSLP) and tumor necrosis factor alpha (TNF-α) are analyzed by enzyme- linked immunosorbent assay. Results: VC lipids mostly contained saturated and branched chains fatty acids. The expression of mRNA and protein of FLG were significantly increased after the supplement with lipid in NHEK cells. Meanwhile, lipids reversed the inhibition of poly (I:C) on FLG. Moreover, lipids suppressed the over secretion of TSLP and TNF-α induced by poly (I:C). Conclusion: These results indicate that lipids extracted from VC has positive effects on the expression of FLG and anti-inflammation, suggesting that lipids of VC may be used for a reference for novel therapeutic method in reducing and remedying skin disease like atopic disease. (Ann Dermatol 31(6) 611∼620, 2019)
-
Yi-Xiang Zhu,WEIWEI HUAN,Ling-yun Yao,Wan-Guo Yu,Ruihua Jiao,Yan-Hua Lu,Renxiang Tan 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.6
Fumigaclavine C (FC), which is produced by Aspergillus fumigatus, is a conidiation-associated ergot alkaloid with significant medical benefits. However, its application is restricted by low yields from submerged cultures. In this study, the technical feasibility of using molasses as a cost-effective ingredient for FC production in a two-stage culture of A. fumigatus was evaluated. The results indicated that molasses supplementation significantly enhanced FC accumulation by promoting conidiation and up-regulating hydroxymethylglutaryl-CoA reductase activity. Via the optimization of the two-stage process in the presence of molasses, FC production in shake flasks reached 226.9 mg/L, which was approximately three times that in the original medium (75.9 mg/L). The use of molasses as a cost-effective ingredient for FC fermentation was also successfully reproduced in a lab-scale bioreactor system in which the maximum FC production reached 215.0 mg/L. The FC production obtained in this study is the highest ever reported. This increased efficiency will enable large-scale production of FC and extend the application of molasses as a low-cost substrate for producing other conidiation-related secondary metabolites.
-
Preparation and properties of Al-Al2O3 metal ceramics via powder metallurgy methods
Jieguang Song,Xiuqin Wang,Lin Chen,Cailiang Pang,Jia Zhang,Weiguo Shi,Songlin Guo,Xianzhong Wang,Ruihua Wang 한양대학교 세라믹연구소 2018 Journal of Ceramic Processing Research Vol.19 No.1
Metal ceramics are outstanding new engineering materials that offer the advantages of both ceramics and metals. In this study,Al-Al2O3 metal ceramics were prepared through powder metallurgy methods, and the densification and properties of theprepared metal ceramics were investigated to gain insights into the preparation of high-performance cermet materials. Resultsrevealed that as the forming pressure was increased, the relative density of the ceramics increased, whereas their electricalresistivity decreased. The relative density increased with increasing sintering temperature up to 700 oC and decreased beyond700 oC, whereas the electrical resistivity exhibited an opposite trend. The relative density increased with increasing Al content,whereas the electrical resistivity displayed an opposite trend. Under optimal technological parameters of 700 oC sinteringtemperature, < 20MPa forming pressure, and 75 wt% Al content, Al-Al2O3 metal ceramics with a high relative density(97.52%) and a low electrical resistivity (101.34 Ω·m) were successfully prepared.
-
Sintering densification and properties of Al2O3/Al cermet materials via powder metallurgy method
Jieguang Song,Yue Liu,Cailiang Pang,Jia Zhang,Lin Chen,Xiling Zhang,Songlin Guo,Xianzhong Wang,Ruihua Wang,Aixia Chen 한양대학교 세라믹연구소 2018 Journal of Ceramic Processing Research Vol.19 No.2
Cermet materials are important new engineering materials with combined advantages of ceramics and metals. In this study,Al2O3/Al cermet materials were prepared through powder metallurgy. The effect of sintering technology on the properties ofAl2O3/Al cermet materials was analyzed as basis for preparing high-performance cermet materials. Results showed that whenthe sintering temperature was increased from 700 oC to 1000 oC under holding time for 1 h, the densification degree and hardnessof Al2O3/Al cermet materials decreased and the electric resistivity increased. In the microstructure of Al2O3/Al cermet materials,Al particles were larger and continuously distributed, whereas the Al2O3 particles were smaller and discontinuously distributed. When the holding time was increased under sintering temperature of 700 oC, the densification degree and hardness of Al2O3/Alcermet materials also increased, and the electric resistivity decreased. When 25 wt% Al2O3/Al cermet materials were sintered at700 oC for 3 h, the densification degree was higher, with hardness of 2203 HV and electric resistivity of 0.0159 Ω • m.
KCI
KISS연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
