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( Khin Lay Kyu ),( Khanok Ratanakhanokchai ),( Patthra Pason ),( Akihiko Kosugi ),( Yutaka Mori ),( Chakrit Tachaapaikoon ),( Chimtong Suphavadee ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.3
An endocellulase-free multienzyme complex was produced by a thermophilic anaerobic bacterium, Thermoanaerobacterium thermosaccharolyticum strain NOI-1, when grown on xylan. The temperature and pH optima for growth were 60℃ and 6.0, respectively. The bacterial cells were found to adhere to insoluble xylan and Avicel. A scanning electron microscopy analysis showed the adhesion of xylan to the cells. An endocellulase-free multienzyme complex was isolated from the crude enzyme of strain NOI-1 by affinity purification on cellulose and Sephacryl S-300 gel filtration. The molecular mass of the multienzyme complex was estimated to be about 1,200 kDa. The multienzyme complex showed one protein on native PAGE, one xylanase on a native zymogram, 21 proteins on SDS-PAGE, and 5 xylanases on a SDS zymogram. The multienzyme complex consisted of xylanase, β-xylosidase, α-L-arabinofuranosidase, β-glucosidase, and cellobiohydrolase. The multienzyme complex was effective in hydrolyzing xylan and corn hulls. This is the first report of an endocellulase-free multienzyme complex produced by a thermophilic anaerobic bacterium, T. thermosaccharolyticum strain NOI-1.
Production and Location of Xylanolytic Enzymes in Alkaliphilic Bacillus sp. K-1
( Yun Sik Lee ),( Khanok Ratanakhanokchai ),( Weela Piyatheerawong ),( Khin Lay Kyu ),( Min Suk Rho ),( Yong Seok Kim ),( Ae Son Om ),( Joo Won Lee ),( Ok Hwa Jhee ),( Ju Seop Kang ),( Gil Hyung Chon 한국미생물생명공학회 2006 Journal of microbiology and biotechnology Vol.16 No.6
Selection of Multienzyme Complex-Producing Bacteria Under Aerobic Cultivation
( Pason Patthra ),( Gil Hyong Chon ),( Khanok Ratanakhanokchai ),( Khin Lay Kyu ),( Ok Hwa Jhee ),( Ju Seop Kang ),( Won Ho Kim ),( Kyung Min Choi ),( Gil Soon Park ),( Jin Sang Lee ),( Hyun Park ),( 한국미생물생명공학회 2006 Journal of microbiology and biotechnology Vol.16 No.8
Purification and Characterization of Two Endoxylanases from an Alkaliphilic Bacillus halodurans C-1
( Tachaapaikoon Chakrit ),( Yun Sik Lee ),( Khanok Rantanakhanokchai ),( Surapong Pinitglang ),( Khin Lay Kyu ),( Min Suk Rho ),( Si Kyung Lee ) 한국미생물생명공학회 2006 Journal of microbiology and biotechnology Vol.16 No.4
Chakrit Tachaapaikoon,Somboon Tanasupawat,Patthra Pason,Somphit Sornyotha,Rattiya Waeonukul,Khin Lay Kyu,Khanok Ratanakhanokchai 한국미생물학회 2012 The journal of microbiology Vol.50 No.3
A mesophilic, facultative, anaerobic, xylanolytic-cellulolytic bacterium, TW1T, was isolated from sludge in an anaerobic digester fed with pineapple waste. Cells stained Gram-positive, were spore-forming, and had the morphology of straight to slightly curved rods. Growth was observed in the temperature range of 30 to 50°C (optimum 37°C) and the pH range of 6.0 to 7.5 (optimum pH 7.0) under aerobic and anaerobic conditions. The strain contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The predominant isoprenoid quinone was menaquinone with seven isoprene units (MK-7). Anteiso-C15:0, iso-C16:0, anteiso-C17:0, and C16:0 were the predominant cellular fatty acids. The G+C content of the DNA was 49.5 mol%. A phylogenetic analysis based on 16S rRNA showed that strain TW1T belonged within the genus Paenibacillus and was closely related to Paenibacillus cellulosilyticus LMG 22232T, P. curdlanolyticus KCTC 3759T, and P. kobensis KCTC 3761T with 97.7, 97.5, and 97.3% sequence similarity, respectively. The DNA-DNA hybridization values between the isolate and type strains of P. cellulosilyticus LMG 22232T, P. curdlanolyticus KCTC 3759T, and P. kobensis KCTC 3761T were found to be 18.6, 18.3, and 18.0%, respectively. The protein and xylanase patterns of strain TW1T were quite different from those of the type strains of closely related Paenibacillus species. On the basis of DNA-DNA relatedness and phenotypic analyses, phylogenetic data and the enzymatic pattern presented in this study, strain TW1T should be classified as a novel species of the genus Paenibacillus, for which the name Paenibacillus xylaniclasticus sp. nov. is proposed. The type strain is TW1T (=NBRC 106381T =KCTC 13719T =TISTR 1914T).