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      저자 : ( Karla I. Cordero-lara ) (검색결과 1 건)
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        Identification of Seedling Vigor-Associated Quantitative Trait Loci in Temperate Japonica Rice

        ( Karla I. Cordero-lara ),( Hyunjung Kim ),( Thomas H. Tai ) 한국육종학회 2016 Plant Breeding and Biotechnology Vol.4 No.4

        A quantitative trait loci (QTL) analysis of seedling vigor traits was conducted under dry-seeded conditions using 176 recombinant inbred lines developed from a cross of two California temperate japonica rice varieties M-203 and M-206. Height at early seedling (HES) and height at late seedling (HLS) stage, growth rate (GR), and fresh weight were evaluated in a growth chamber. Assessment of HES, HLS, and GR was also performed in outdoor basins in 2015 and 2016. M-203 was significantly better for all traits in the growth chamber, however, no significant differences were observed between the parents in the outdoor basins with the exception of HES in 2015. Genotyping by sequencing was used to construct a map of >6,000 single nucleotide polymorphisms. Six QTL were detected in the growth chamber, one each on chromosomes 2, 9, 10, and 12 and two at the same position on 1. qFW1 and qHES1 explain 34.6% and 8.7% of the phenotypic variation observed for their respective traits and correspond to previously reported QTL for shoot length. All positive effects were contributed by the M-203 allele except for the qHLS12. Two QTL, qHES8 (12.3%; M-206 allele) and qHES9 (9.4%; M-203 allele), were detected in the outdoor basin 2016, but none in 2015. RIL-187 was consistently among the highest vigor lines in all environments and additional evaluations confirmed this finding. The molecular and genetic resources developed here will facilitate further investigation of seedling vigor and breeding of enhanced temperate japonica rice varieties.

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