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( Myung-a Jung ),( Jawon Shin ),( Ara Jo ),( Huwon Kang ),( Gyuok Lee ),( Dool-ri Oh ),( Hyo Jeong Yun ),( Sojeong Im ),( Donghyuck Bae ),( Jaeyong Kim ),( Chul-yung Choi ) 한국응용생명화학회(구 한국농화학회) 2020 Journal of Applied Biological Chemistry (J. Appl. Vol.63 No.4
Testosterone deficiency syndrome (TDS), also known as late-onset hypogonadism, is a clinical and biochemical syndrome associated with advanced age and characterized by deficient serum testosterone levels. The Elaeagnus multiflora fruit (EMF) and Cynanchum wilfordii (CW) have been used in traditional herbal medicine. This study aimed to investigate the therapeutic effects of EMF and CW mixtures (at the ratios of 3:7, 5:5, and 7:3) on TDS using TM3 cells and aging male rats. EMF, and mixtures of EMF and CW (at the ratios of 3:7, 5:5, and 7:3) significantly increased testosterone levels in TM3 cells (p <0.05). The rats were orally administered EMCW (EMF and CW mixed at the ratio of 3:7 50, 100 and 200 mg/kg/day) for 4 weeks consecutively. After 4 weeks of EMCW administration, latency time on the rotarod test, and serum testosterone and dehydroepiandrosterone sulfate levels were significantly increased (p <0.05 and p <0.01). Moreover, the levels of globulin-bound sex hormones were decreased in the EMCW-fed groups. However, prostate-specific antigen levels did not differ among the groups. These results suggest that EMCW can be effectively used to alleviate TDS.
Anti-fatigue effects of Elaeagnus multiflora fruit extracts in mice
( Myung-a Jung ),( Ara Jo ),( Jawon Shin ),( Huwon Kang ),( Yujin Kim ),( Dool-ri Oh ),( Chul-yung Choi ) 한국응용생명화학회(구 한국농화학회) 2020 Journal of Applied Biological Chemistry (J. Appl. Vol.63 No.1
The fruit, leaves, and roots of Elaeagnus multiflora Thunb. have been used in traditional Chinese medicine to treat cough, diarrhea, and itching. However, the anti-fatigue effects of E. multiflora fruit (EMF) extract have not been studied in detail. The aim of this study was to examine the effect of EMF on fatigue and exercise performance in BALB/c mice. EMF was orally administered to mice at four doses (10, 50, 100, and 200 mg/kg/ day) for 2 weeks. The anti-fatigue activity was evaluated by determining the exhaustive swimming time. Blood lactate and glucose levels and serum lactate levels after a 10 min swimming time, as well as ammonia, creatine kinase (CK), blood urea nitrogen (BUN), lactate dehydrogenase (LDH), and glycogen contents after exhaustive swimming time were measured. The exhaustive swimming time of the EMF 200 group was significantly increased (p <0.01). The EMF groups showed significantly low levels of CK, BUN, LDH, and lactate compared with the control group (p <0.05). Increased liver glycogen was observed in the EMF 200 group (p <0.05). These results suggest that EMF can be utilized as an efficacious natural resource for its anti-fatigue effects.
Oh, Dool-Ri,Kim, Yujin,Choi, Eun-Jin,Jo, Ara,Shin, Jawon,Kang, Huwon,Lee, Seul-Gi,Kim, Jaeyong,Kim, Young Ran,Choi, Chul Yung World Scientific Publishing Company 2018 The American journal of Chinese medicine Vol.46 No.7
<P>The present study evaluates the anti-oxidative stress activity of <I>Vaccinium bracteatum</I> Thunb. fruit extract (VBFW) to identify the mechanisms responsible for its antidepressant-like effects. To evaluate the antidepressant and anti-oxidant effects of VBFW, malondialdehyde (MDA), serotonin transporter (SERT), and monoamine oxidase A (MAO-A) levels were measured in a mouse model of chronic restraint stress (CRS). The underlying mechanisms preventing oxidative stress and neuronal apoptosis were investigated using <I>in vitro</I> models of hydrogen peroxide (H<SUB>2</SUB>O<TEX>$ _{2})$</TEX>-induced neuronal damage. The results showed that VBFW treatment (200<TEX>$ \,$</TEX>mg/kg) significantly reduced MDA, SERT, and MAO-A levels in the prefrontal cortex of CRS mice. Furthermore, VBFW (30<TEX>$ \,$</TEX><TEX>$ \mu $</TEX>g/mL) exhibited protective effects against H<SUB>2</SUB>O<SUB>2</SUB>-induced cell death via inhibition of the H<SUB>2</SUB>O<SUB>2</SUB>-induced increase in Bax and decrease in Bcl-2 levels within the mitochondria of SH-SY5Y cells. Furthermore, VBFW (10 and 30<TEX>$ \,$</TEX><TEX>$ \mu $</TEX>g/mL) exerted protective effects against H<SUB>2</SUB>O<SUB>2</SUB>-induced cell death through inhibition of key mitochondria-associated apoptotic proteins such as cytochrome c, caspase-3 and PARP. Additionally, VBFW (10 and 30<TEX>$ \,$</TEX><TEX>$ \mu $</TEX>g/mL) could improve the activity of anti-oxidant enzymes (such as SOD and catalase) in H<SUB>2</SUB>O<SUB>2</SUB>-treated SH-SY5Y cells. These results suggest that the antidepressant and anti-oxidant effects of VBFW might be mediated by the regulation of SERT and MAO-A, and possibly associated with regulation of oxidative stress-induced apoptosis.</P>
Oh, Dool-Ri,Kim, Yujin,Choi, Eun-Jin,Jung, Myung-A,Oh, Kyo-Nyeo,Hong, Ji-Ae,Bae, Donghyuck,Kim, Kwangsu,Kang, Huwon,Kim, Jaeyong,Kim, Young Ran,Cho, Seung Sik,Choi, Chul-Young World Scientific Publishing Company 2018 The American journal of Chinese medicine Vol.46 No.2
<P>The fruit of <I>Vaccinium bracteatum</I> Thunb. (VBF) is commonly known as the oriental blueberry in Korea. The aim of this study was to evaluate the antidepressant-like effects of water VBF extract (VBFW) in a mouse model of chronic restraint stress (CRS) and to identify the underlying mechanisms of its action. The behavioral effects of VBFW were assessed in the forced swim test (FST) and open field test (OFT). The levels of serum corticosterone (CORT), brain monoamines, in addition to the extracellular signal-regulated kinases (ERKs)/protein kinase B (Akt) signaling pathway were evaluated. VBFW treatment significantly reduced the immobility time and increased swimming time in FST without altering the locomotor activity in unstressed mice. Furthermore, CRS mice treated with VBFW exhibited a significantly decreased immobility time in FST and serum CORT, increased locomotor activity in OFT, and enhanced brain monoamine neurotransmitters. Similarly, VBFW significantly upregulated the ERKs/Akt signaling pathway in the hippocampus and PFC. In addition, VBFW may reverse CORT-induced cell death by enhancing cyclic AMP-responsive element-binding protein expression through the up-regulation of ERKs/Akt signaling pathways. In addition, VBFW showed the strong antagonistic effect of the 5-HT<TEX>$ _{2\text{A}}$</TEX> receptor by inhibiting 5-HT-induced intracellular Ca<TEX>$ ^{2+}$</TEX> and ERK1/2 phosphorylation. Our study provides evidence that antidepressant-like effects of VBFW might be mediated by the regulation of monoaminergic systems and glucocorticoids, which is possibly associated with neuroprotective effects and antagonism of 5-HT<TEX>$ _{2\text{A}}$</TEX> receptor.</P>