http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : ( Ekrem Gurel ) (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
-
-
Ozturk, Mehmet,Gurel, Ekrem,Watmough, Nicholas J.,Mandaci, Sevnur Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.5
Cytochrome $cbb_3$ oxidase is a member of the heme-copper oxidase superfamily that catalyses the reduction of molecular oxygen to the water and conserves the liberated energy in the form of a proton gradient. Comparison of the amino acid sequences of subunit I from different classes of heme-copper oxidases showed that transmembrane helix VIII and the loop between transmembrane helices IX and X contain five highly conserved polar residues; Ser333, Ser340, Thr350, Asn390 and Thr394. To determine the relationship between these conserved amino acids and the activity and assembly of the $cbb_3$ oxidase in Rhodobacter capsulatus, each of these five conserved amino acids was substituted for alanine by site-directed mutagenesis. The effects of these mutations on catalytic activity were determined using a NADI plate assay and by measurements of the rate of oxygen consumption. The consequence of these mutations for the structural integrity of the $cbb_3$ oxidase was determined by SDS-PAGE analysis of chromatophore membranes followed by TMBZ staining. The results indicate that the Asn390Ala mutation led to a complete loss of enzyme activity and that the Ser333Ala mutation decreased the activity significantly. The remaining mutants cause a partial loss of catalytic activity. All of the mutant enzymes, except Asn390Ala, were apparently correctly assembled and stable in the membrane of the R. capsulatus.
-
Verma Sandeep Kumar,Gantait Saikat,Mukherjee Epsita,Gurel Ekrem 한국작물학회 2022 Journal of crop science and biotechnology Vol.25 No.5
Lycium barbarum (Solanaceae), commonly known as Goji (or wolfberry), is popular for its nutritive and medicinal properties and is called a “super fruit” or “super food”. Considering the importance of the economic utility, the potential of different explants (hypocotyl, leaf and root) of L. barbarumfor plant regeneration and somatic embryogenesis has been evaluated in the present study. Two sets of experiments were carried out; the first compared Murashige and Skoog (MS) medium suplemented with different concentrations of 2,4-dichlorophenoxy acetic acid (2,4-D), N6- benzylaminopurine (BAP), thidiazuron (TDZ), kinetin, and zeatin alone, while the second set tested the combinations of TDZ with 2,4-D to inducecallus and subsequent shoot or embryo formation, respectively. For callus and subsequent shoot induction (for the first set of experiment), 2,4-D was the most effective for callus induction (100%), while TDZ at 1 mg/L produced a mean of 5 shoots per callus. For the second set of experiment spontaneous induction of somatic embryos as well as subsequent maximum shoot regeneration was recorded as 0.25 mg/L 2,4-D and 1 mg/L TDZ supplemented medium. Hypocotyl explant proved to be the most responsive organ that induced as many as 6 somatic embryos and subsequent 9regenerated shoots per callus. Later, the plantlets were successfully acclimatized (100%) and finally transferred to the greenhouse. Total phenolic content was measured from shoot, brown callus and white callus of L. barbarum L. grown in MS medium supplemented with auxin alone (for callus) and auxin-cytokinin (for shoots). The highest amount of total phenolic content (640 mg GAE/g DW) was obtained in shoot. The described protocol provides a simple way to regenerate plants through direct and indirect organogenesis as well as somatic embryogenesis, which would be useful for mass propagation, large-scale production of secondary metabolites, germplasm conservation, and genetic transformation studies in this medicinally important species.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
