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Genetic transformation of wheat: current status and future prospects
Jiarui Li,Xingguo Ye,Baoyan An,Lipu Du,Huijun Xu 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.3
Genetic transformation is a reverse genetics tool for validation of target genes and crop improvement. However, due to its low efficiency and genotype dependency,wheat is considered a recalcitrant plant for genetic transformation. During the last 20 years, various in vitro and in planta transformation methods have been reported in wheat. Until now, biolistic particle and Agrobacteriummediated wheat transformation methods using immature embryos as explants have been the two major transformation approaches. In addition to immature embryos, other explant types, such as mature embryos, anther-derived calli, inflorescences, apical meristems, and other floral organs, have been employed; however, they need further optimization. In addition to the common marker genes,such as bar, hpt and gus, other effective markers, ALS,AtMYB12 and pmi, have been successfully used for selection of positive transgenic plants. Numerous agronomic trait genes such as biotic stress resistance or tolerance genes have been transferred into wheat plants. Future prospects, such as recipient wheat cultivars and explants,marker free issues, and transgene silencing, are discussed. The objective of this review is to summarize current successful techniques for wheat transformation and stimulate further research into long-term wheat improvement by genetic engineering approaches.
( Yuepeng Song ),( Miaomiao Chen ),( Baoyan Xu ),( Dongsheng Gao ),( Jing Guo ),( Lingfeng Xu ),( Zheng Wang ),( Hyoung Seop Kim ) 대한금속재료학회(구 대한금속학회) 2016 대한금속·재료학회지 Vol.54 No.11
Herein, we report the results of our investigation on the effect of friction and anvil design on the heterogeneous plastic-deformation characteristics of copper during the compressive stage of high-pressure torsion (HPT), using the finite element method. The results indicate that the friction and anvil geometry play important roles in the homogeneity of the deformation. These variables affect the heterogeneous level of strain in the HPT compressed disks, as well as the flash in the disk edge region. The heterogeneous plastic deformation of the disks becomes more severe with the increasing depth of the cavity, as anvil angle and friction coefficient increase. However, the homogeneity increases with increases in the wall angle. The length of flash and the area of the dead metal zone increase with the depth of the cavity, while they decrease at a wall angle of 180°. (Received March 17, 2016; Accepted May 25, 2016)
Inhomogeneous Deformation of Interstitial Free Steel during the High Pressure Torsion Process
Yuepeng Song,Miaomiao Chen,Wenke Wang,Baoyan Xu,Dongsheng Gao,Shuai Zhang,Hyoung Seop Kim 대한금속ㆍ재료학회 2017 대한금속·재료학회지 Vol.55 No.10
Interstitial free (IF) steel disks were subjected to various degrees of revolution during application of the high-pressure torsion (HPT) process, and the resulting distributions of hardness and microstructure during the early torsion stage of high-pressure torsion (HPT) were investigated using experimental and simulation approaches. The results indicated that the deformation in the HPT-processed IF steel disk was inhomogeneous, producing low hardness in the center and high hardness in the edge region. The experimental results, including the hardness and microstructure distributions, indicated that the severe deformation zone proceeds gradually from the center to the edge of the HPT disks in the early torsion stage, and also confirmed verify that the deformation on the upper surface of the disks lags behind that on the bottom surface. Simulation results from a finite element method analysis strongly supported the experimental conclusions. (Received December 30, 2016; Accepted July 2, 2017)