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      • DYNAMICS OF HELMINTHIC INFECTIONS IN EPIDEMIC AND EPIZOOTIC SYSTEMS OF BANGLACESH

        TILAK CHANDRA NATH 충북대학교 2022 국내박사

        RANK : 233023

        In view of a growing national and international commitment to control human helminths infection, there is an urgent need to intensify the cases detection from various reservoir hosts and the application of context-based diagnostic methods. The goal of this study was to deepen the understanding of helminthiases, perform a One Health based parasitological screening comprising humans, animals, and environmental samples from same ecological settings and assess feasibility of a diagnostic tool and control intervention in Bangladesh. The dissertation employed a combination of qualitative and quantitative methods including a series of parasitological techniques like copromicroscopy, scanning electron microscopy, experimental sedation, culture and molecular approach. Specimens (stools, soils, and adult helminths) were collected from humans, animals, and environment from the same communities (Dhaka, Chittagong and Chattogram) in Bangladesh. Structured questionnaires and an interventional study were used to collect data. Interviews were conducted among community people and related professionals. The examined host communities harbored diverse types of helminths that were heterogeneously distributed across the hosts. Out of 360 human stool samples, the prevalence of helminths infection was 31.7%. Interventional study showed that integration of new intervention with existing control programs was feasible in the local field settings of the country; however, several barriers like fragmented health delivery system, lack of interdisciplinary communication, and information gaps were dominantly existing. Additionally, this study presents the first human case of Rhabditis sp. infection in Bangladesh; emphasized for careful inspection to diagnose and differentiate the helminths species. Out of 550 fecal samples from animal, 59.6% was found positive for at-least one helminth. This study suggested a new, undescribed nematode, Agriostomum n. sp. (2021) from Black Bangal goat. In captive wild animals, 48.9% of examined fecal samples were found positive for parasitic infections. For the first time, zoonotically important Spirometra decipiens from lion was molecularly characterized in this study. Furthermore, geohelminth contamination was found in 52.5% of examined soil samples. This study also developed a context-based geohelminth diagnostic method and the method found feasible in terms of field applicability and egg recovery rate. And lastly, this study molecularly identified zoonotically important canine hookworms, Ancylstoma caninum from soil that represents a risk to public and animal health. This study assessed the prevalence of helminth infection in humen, animal, wild animal as well as geohelminth contamination, and provides a general overview of host-parasite interactions, diagnostic tools and control strategies of parasitic diseases in Bangladesh. Helminthiases remains dominant among the diverse host range, with a high rate of geohelminth contamination. The need of routine parasitological examination as part of current parasite control programs is highlighted in this study. Armed with this, the study propose One Health approach, integrating human, veterinary, and public health authorities in the development and implementation of parasitic diseases prevention and control strategies.

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