관절염 흰쥐모델에서 COX-2 억제제가 관절통과 척수의 SP와 CGRP 발현에 미치는 영향 = Effect of COX-2 inhibitor on the arthritic pain and the expression of spinal SP and CGRP in arthritic rat

다국어 초록 (Multilingual Abstract)

Substance P(SP) and Calcitonin-gene related peptide(CGRP), the most well known pain-related neuropeptides, are known to be increased in experimental arthritis. However, in experimental arthritic rat, mechanisms underling analgesic action of COX-2 inhibitor and the relationship between changes of SP and CGRP expression in spinal cord level and the changes of the pain remains yet unclear.
The present study was to investigate the effects of cyclooxygenase-2 (COX-2) inhibitor NS-398 on the changes of SP and CGRP expression in the spinal cord and pain-related behavior in experimental arthritic rat model.
The arthritis was induced by carrageenan (2% in saline, 50㎕)injection into the knee joint cavity. Pain-related behavior (i.e., decrease in weight load in the affected leg) and the expression of spinal SP and CGRP by immunohistochemical analysis were measured.
Injection of carrageenan into the knee joint cavity resulted in a significant decrease of weight load and increase of SP and CGRP expression in the dorsal horn of the L4 and L5 level. However, pre-treatment of NS-398 (5mg/kg, 1 ㎖) prevented the reduction of weight load of carrageenan-injected leg and post-treatment of NS-398 partially reversed the reduction of weight load induced by carrageenan injection. Spinal SP and CGRP immunoreactivitys in the spinal cord were reduced following pre- and post-treatment of NS-398, except for SP expression in L5. These results suggest that the analgesic effect of COX-2 inhibitor may arise via modulating the expression of spinal SP and CGRP.

목차 (Table of Contents)

• 목차
• 초록
• 서론 = 1
• 연구재료 및 방법 = 4
• 1. 실험동물 및 염증의 유발 = 4
• 2. 행동학적 검사 (Weight bearing test) = 4
• 2.1 실험 장치 = 4
• 2.2 약물처치 및 검사절차 = 8
• 3. 면역조직화학 검사 = 8
• 4. 면역조직화학염색법의 과정 = 9
• 5. 자료 분석 및 통계처리 = 10
• 결과 = 11
• 1. 행동학적 변화 = 11
• 2. 4번 요수에서의 SP에 대한 COX-2 억제제의 효과 = 16
• 3. 5번 요수에서의 SP에 대한 COX-2 억제제의 효과 = 19
• 4. 4번 요수에서의 CGRP에 대한 COX-2 억제제의 효과 = 22
• 5. 5번 요수에서의 CGRP에 대한 COX-2 억제제의 효과 = 25
• 논의 = 28
• 참고문헌 = 33