Engineering of Glycerol Monostearate Lipid- Based Nanocarriers

다국어 초록 (Multilingual Abstract)

The limitations of quercetin, a polyphenol flavonoid with antioxidants, antibacterial, and wound healing properties but poor stability and solubility in aqueous media, were overcome by using a modified hot homogenization method to improve the physicochemical and biological performance of quercetin-loaded solid lipid nanoparticles （SLNQ）. The optimized formulation （SLNQ4） exhibited high colloidal stability, a particle size of ~220 nm, a narrow size distribution, a moderate negative surface charge （-21.50 mV）, and high drug entrapment （〉96%）. Uniform, smooth, and semi-spherical nanoparticles were formed as evidenced by FE-SEM analysis. The in vitro release assay showed a biphasic profile with sustained drug release and indicated the best fit to the Higuchi model （R2 = 0.986） for diffusion-controlled release from the drug-enriched lipid matrix core. SLNQ4 not only retained strong antioxidant activity but also showed antibacterial activity against a wide range of Gram-positive and Gram-negative bacteria. Improved wound closure and tissue healing/regeneration were also observed in an in vivo wound-healing assay with approximately 88% and 99% of wound closure on days 10 and 15, respectively. The increased therapeutic efficacy was attributed to improved quercetin stability, sustained release, prolonged retention at the wound site, and preserved biological activity. All in all, the data indicates that SLNQ4 is a promising, multifunctional nanocarrier that addresses the drawbacks of free quercetin, thereby enhancing its therapeutic potential for dermatological, pharmacological, and wound-healing treatments.

목차 (Table of Contents)

• 1.1. Introduction 1
• Chapter II. Materials and Method 5
• 2.1. Materials 6
• 2.2. Preparation for SLNQ fabrication 6
• 2.3. Size analysis and polydispersity index 6
• 2.4. Zeta potential 6
• 2.5. Long-term physical stability study 7
• 2.6. Encapsulation efficiency 7
• 2.7. Scanning electron microscopy (SEM) 7
• 2.8. Drug release assay 7
• 2.9. Antioxidant activity test (DPPH assay) 8
• 2.10. Antibacterial Disk Diffusion Assay 8
• 2.11. Antibacterial Growth Curve OD600 9
• 2.12. In Vivo Wound Healing Study 9
• Chapter III. Results and Discussion 11
• 3.1. Preparation for SLNQ fabrication 12
• 3.1.1. Determination of surfactant ratio 13
• 3.1.2. Determination of homogenization time and speed 18
• 3.2. Scanning electron microscopy (SEM) 22
• 3.3. Long-term physical stability study 26
• 3.4. In vitro release studies 29
• 3.5. Antioxidant assay 35
• 3.6. Antibacterial Assay 38
• 3.6.1. Disc Diffusion Assay 38
• 3.6.2. OD600 growth curve assay 42
• 3.7. In Vivo wound healing assay 46
• Chapter IV. Conclusion 52
• References 57
• CURRICULUM VITAE 78
• Education 78
• Publication 78
• Acknowledgements 79