Mealworms (Tenebrio molitor larvae) are considered a sustainable food source due to their nutritional, industrial, and environmental advantages. However, their characteristic appearance and odor can negatively influence consumer acceptance. Lactic aci...
Mealworms (Tenebrio molitor larvae) are considered a sustainable food source due to their nutritional, industrial, and environmental advantages. However, their characteristic appearance and odor can negatively influence consumer acceptance. Lactic acid fermentation is a promising approach to improve sensory properties and functionality of insect-based foods. In this study, mealworms were fermented using commercial lactic acid bacteria (LAB) starters (ABY-3, CH-1, CHN-11, FLORA DANICA, LH-B02, R-707, ST-BODY-1, TCC-4, YC-380, and YF-L812). Fermentation was conducted for up to 5 days, and physicochemical properties, LAB viability, hydrolysis degree, bioactive compounds, and antioxidant activities were evaluated. After 3 days of fermentation, most samples showed decreased pH and sugar content, while titratable acidity and crude protein content increased. LAB counts exceeded 7.0 log CFU/g in CHN-11, TCC-4, and YC-380, and hydrolysis degree was higher in LH-B02, ST-BODY-1, TCC-4, and YC-380 compared to the control. ST-BODY-1 and TCC-4 increased total polyphenol (3.21–3.39 GAE mg/g) and flavonoid contents, while antioxidant activities varied depending on the starter. During 5-day fermentation, pH continued to decrease, titratable acidity and hydrolysis degree peaked at day 5, and LAB counts reached 6.52–8.12 log CFU/g on day 2 before declining. TCC-4 maintained viable counts above 7 log CFU/g and exhibited the highest polyphenol (8.49 GAE mg/g) and flavonoid (1.75 QE mg/g) levels on day 4. SOD-like activity increased to 50.60–56.16% by day 5. These findings suggest that specific starters, particularly TCC-4, exhibit superior fermentation suitability by enhancing LAB growth, hydrolysis, bioactive compounds, and antioxidant activity. Therefore, TCC-4 and selected LAB strains are promising candidates for developing functional and palatable fermented mealworm products.