This study was conducted to evaluate the effects of beneficial microorganisms—Bacillus subtilis, Lactobacillus casei, and Lactobacillus plantarum—on the germination and growth of water spinach (Ipomoea aquatica F.). Water spinach seeds were soaked...
This study was conducted to evaluate the effects of beneficial microorganisms—Bacillus subtilis, Lactobacillus casei, and Lactobacillus plantarum—on the germination and growth of water spinach (Ipomoea aquatica F.). Water spinach seeds were soaked in a 1:100 dilution of beneficial microorganisms for 4 hours, after which germination experiments were conducted for 7 days and growth experiments for 6 weeks as a single treatment. The growth experiments were carried out on a cocopeat medium, and nutrient solution was periodically supplied during the cultivation period. In the germination experiment, germination percentage, germination time, and germination speed were measured, while in the growth experiment plant height, leaf length, leaf width, number of leaves, fresh weight, and dry weight were investigated. The experimental results showed that the beneficial microorganism treatment groups exhibited no significant differences in germination compared to the control group. However, in the growth experiment of water spinach (Ipomoea aquatica F.), the lactic acid bacteria treatment group at week 3 showed significant differences in chlorophyll content (SPAD), fresh weight, and particularly dry weight compared to the control group. The Bacillus subtilis treatment group at week 5 showed significant differences in root length, shoot length, and dry weight. These results indicate that beneficial microorganisms influenced the early growth of water spinach (Ipomoea aquatica F.), while Bacillus subtilis was advantageous for long-term growth. This experiment can serve as basic data for the development of hydroponic cultivation technologies and may also contribute to expanding hydroponic research to subtropical crops such as water spinach (Ipomoea aquatica F.) in preparation for climate change, as well as extending the potential application of beneficial microorganisms used in soil cultivation to soilless cultivation.