Point-of-care technology for SNP detection with polymerase chain reaction

다국어 초록 (Multilingual Abstract)

Single nucleotide polymorphism (SNP) indicates the differences of a nucleotide in the genetic sequences among the population. SNP is a biomarker for the significant traits of an individual, and it also causes hereditary diseases related to neurodegenerative, cancer, or chromosomal disorders. Real-time polymerase chain reaction (RT-PCR) is the gold standard for SNP identification due to its excellent accuracy and speed. However, RT-PCR needs expensive consumables for the reaction as well as a compact machine. In this research, the SNP genotyping for ranavirus and Alzheimer’s disease is performed with conventional PCR and colorimetric detection for the output visualization. The ranavirus strains are obtained in different size bands, and the Alzheimer’s polymorphism can be distinguished with quick amplification. The overall time for the amplification and visualization step is under 40 min.

목차 (Table of Contents)

• 1. Introduction 1
• 2. Method 4
• 2.1. Chemical and Material 4
• 2.2. Data gathering and gene analysis 5
• 2.3. PCR assay for ranavirus detection 6
• 2.4. PCR assay for APOE detection 8
• 2.5. Colorimetric detection with silver nitrate 10
• 3. Result and Discussion 11
• 3.1. Data collection and analysis 11
• 3.2. Detection of ranavirus strains with AS-PCR 16
• 3.3. Detection of APOE polymorphism with AS-PCR 19
• 3.4. Visualization for the PCR amplification 23
• 4. Conclusion 26
• References 27