벚나무류(Prunus spp.)에서 발생한 고약병(Felt Disease)의 형태 및 분자다양성 분석 = Morphological Characteristics and Molecular Diversity of Felt Disease on Prunus spp.

다국어 초록 (Multilingual Abstract)

This study aimed to investigate the diversity of felt disease on domestic Prunus species by conducting morphological and molecular analyses. A total of 19 fruiting bodies of felt disease were collected from 11 regions in Korea, including Gyeongbuk, Gyeongnam, Jeonbuk, Jeonnam, and Jeju Island. The collected fruiting bodies underwent comprehensive analysis, including observation of external morphological changes, examination of internal and external structures, analysis of basidiospore characteristics, and ITS rDNA-based phylogenetic analysis. Additionally, we identified symbiotic insects.
The fruiting bodies of the felt disease exhibited distinct seasonal color changes, tending to appear relatively light during spring and summer and gradually darkening as autumn approached. This suggests that color is not a fixed trait of the felt disease fruiting body but can vary depending on environmental conditions or developmental stage. This indicates the need to reexamine the validity of color-based classification names commonly used domestically.
Cross-sectional analysis of the fruiting bodies revealed distinct tissue layers: subiculum, pillars, subhymenial layer, and hymenial layer. Based on the combination and arrangement of these layers, four morphological types were observed. Each type differed in the arrangement, regularity, overlap, and degree of development of the layers. The hyphal diameter was consistently approximately 2 μm. While no morphological variation in the hyphae themselves was observed, individual variation was noted based on the fruiting body's color and the degree of hyphal overlap.
Phylogenetic analysis based on ITS rDNA identified four clades that corresponded to the four groups confirmed by RFLP analysis. The external morphology, the internal and external structure of the fruiting body, the RFLP patterns, and the ITS rDNA clades all tended to cluster into the same groups. This indicates that morphological characteristics and molecular diversity are expressed consistently. Basidiospore morphology was observed in only some specimens, but all measured values fell within the range reported for the Septobasidium genus in previous studies. Analysis of symbiotic insects confirmed the presence of Pseudaulacaspis prunicola in all fruiting bodies. This suggests a possible species-specific relationship between the host plant, symbiotic insect, and felt disease.
This study confirmed that the felt disease affecting Prunus spp. in southern Korea consists of genetically distinct lineages rather than simple morphological variation. These findings underscore the importance of comprehensive research that combines morphological, molecular, and ecological approaches in future felt disease studies. They are also expected to serve as foundational data for Septobasidium research in Korea.

목차 (Table of Contents)

• Ⅰ. 서론 1
• Ⅱ. 재료 및 방법 4
• 1. 연구 대상 및 자실체 시료 확보 4
• 2. 고약병 자실체 외부 형태 특성 7
• 3. 고약병 자실체 구조 특성 10
• 4. 담자포자 및 균사 미세구조 분석 11
• 5. Total DNA 추출 12
• 6. ITS rDNA 영역 PCR 증폭 14
• 7. ITS rDNA 염기서열 분석 16
• 8. ITS 기반 제한효소 절편길이 다형성(RFLP) 분석 17
• 9. 고약병 내 깍지벌레 관찰 및 분석 19
• Ⅲ. 결 과 23
• 1. 채집 자실체 형태 특성 23
• 2. 자실체 외부 형태 변화 27
• 3. 자실체 표면 구조의 형태적 특징 34
• 4. 자실체 단면 구조의 층위별 특성 37
• 5. 담자포자 형태 특징 43
• 6. 자실체 층위별 균사 구조 특성 46
• 7. ITS rDNA 영역 PCR 증폭 48
• 8. ITS rDNA 영역 PCR-RFLP 49
• 9. ITS rDNA 영역 계통수 분석 53
• 10. 고약병 자실체 내 공생 깍지벌레의 형태 및 COI 분석 56
• Ⅳ. 고찰 61
• Ⅴ. 결론 63
• Ⅵ.참고문헌 64