Augmenting Efficacy of Cell Therapy through Integration with Physical Stimulation-Based Biomedical Devices = 물리적 자극 기반 바이오 메디컬 장치를 이용한 세포치료제 효율 증진 연구

다국어 초록 (Multilingual Abstract)

Conventional cell therapies in regenerative medicine encounter significant challenges, including inefficient delivery systems, poor cell survival, and limited therapeutic effects. Mesenchymal stem cells (MSCs) are recognized for their therapeutic potential due to their immunomodulatory and anti-inflammatory properties. To address the limitations of cell therapy, this study investigates various strategies to enhance its efficacy by integrating advanced biomedical devices and physical stimulation techniques. Initially, the research focuses on an acoustic pressure system for the rapid formation of heterotypic pseudo-islets using adipose-derived stem cells, aiming to improve graft survival in pancreatic islet transplantation. Subsequently, a patch system incorporating gold nanoturf is introduced for wireless photothermal upregulation of stem cell spheroids and multi-dimensional cell sheet formation, enhancing wound healing through synergistic skin-wound closure. Finally, an extrusion-based system is described for generating nanovesicles from stem cells under light irradiation and hypoxia, aiming to rejuvenate fibroblast function. These studies demonstrate the potential of integrating physical stimulation with cell-based therapies to overcome existing limitations, thereby enhancing therapeutic outcomes in tissue regeneration. The research underscores the synergistic benefits of combining advanced bioengineering strategies with cell therapies, paving the way for next-generation regenerative medicine technologies with improved clinical translation potential.

목차 (Table of Contents)

• Chapter 1. Introduction 1
• 1.1. Introduction 1
• 1.2. Object of the thesis 4
• Chapter 2. Shortening the Period of Spheroid Formation with Acoustic Pressure 7
• Subaqueous acoustic pressure system based one day heterotypic pseudo-islet spheroid formation with adipose derived stem cells for graft survival-related function enhancement 7
• 2.1 Introduction 7
• 2.2 Result and Discussion 10
• 2.3 Experimental Section 25
• 2.4 Conclusion 36
• Chapter 3. Enhancing Therapeutic Effect and Delivery Efficiency of Spheroid with Patch System 37
• 3.1 Gold Nanoturf-Mediated Wireless Photothermal Upregulation of Human Adipose Derived Stem Cell Spheroids for Synergistic Skin-Wound Closure 37
• 3.1.1 Introduction 37
• 3.1.2 Result and Discussion 41
• 3.1.3 Experimental Section 72
• 3.1.4 Conclusion 89
• 3.2 Facile Size Tunable Skin-Adaptive Patch for Accelerating Wound Healing 92
• 3.2.1 Introduction 92
• 3.2.2 Result and Discussion 97
• 3.2.3 Experimental Section 114
• 3.2.4 Conclusion 126
• Chapter 4. Stem Cell Nanovesicle Formation with Extrusion System 160
• 4.1 Fibroblast function recovery through rejuvenation effect of nanovesicles extracted from human adipose-derived stem cells irradiated with red light 129
• 4.1.1 Introduction 129
• 4.1.2 Result and Discussion 132
• 4.1.3 Experimental Section 154
• 4.1.4 Conclusion 166
• 4.2 Hypoxia potentiated pro-angiogenic and anti-fibrotic effect of nanovesicles derived from human mesenchymal stem cells 167
• 4.2.1 Introduction 167
• 4.2.2 Result and Discussion 169
• 4.2.3 Experimental Section 184
• 4.2.4 Conclusion 194
• Chapter 5. Concluding remarks 196
• Chapter 6. References 198
• 논문 요약 253