Encephalomyocarditis virus 표면항원의 단일항체생산 세포주의 크론과 이의 면역학 및 생화학적 연구 (Ⅰ) = Cloning of Monoclonal Antibody-Producing Cell Lines to Encephalo-myocarditis Virus Surface Antigen and Immunological and Biochemical Properties of the Monoclonal Antibody (Ⅰ)

다국어 초록 (Multilingual Abstract)

To determine the immunological and biochemical properties of monoclonal antibody against encephalomyocarditis viral surface antigen , Balb/c female mice were immunized with the purified viral surface protein of D-variant of encephalomyocarditis virus and then fused with myeloma cells (NS-1). Sixteen monoclonal antibody-producing hybridoma cell lines were selected from non-producing hybridoma cell lines. Among them, three hybridoma cell lines. Among them, three hybridoma cell lines produced IgG2b and other thirteen hybridoma cell lines produced IgGI . Two hybridoma cell lines, EDH- S- 23 and EDH - S- 24 , produced IgG2b and showed highest neutralization titer, 256. The antigenic specificity of these monoclonal antibodies was specific to D - variant of encephalomyocarditis virus . Moreover, the monoclonal antibody showed specifically the curring effect on diabetes mellitus of mice, which was induced by the D - variant of encephalomyocarditis virus.

목차 (Table of Contents)

• 목차 = 1
• ABSTRACT = 3
• 서론 = 4
• 실험재료 및 방법 = 6
• 결과 및 고찰 = 10
• 결론 = 15
• 참고문헌 = 16