Xanthohumol에 의한 MDA-MB231 유방암세포주의 세포자멸사 유발 기전 = Xanthohumol induces apoptotic cell death of MDA-MB231 breast cancer cells

다국어 초록 (Multilingual Abstract)

In this study, I used MDA-MB231 cells, a human breast cancer cell line to investigate the effect of xanthohumol on cell viability. Treatment of MDA-MB231 cells with xanthohumol caused apoptotic cell death in a concentration and a dose-dependent manners. The molecular mechanism by which xanthohumol induces apoptosis appeared to involve the release of cytochrome c from mitochondria to cytoplasm with concurrent increase of Bax level in the mitochondria. The increase of cytochrome c concentration in the cytoplasm resulted in apoptosis through activation of caspase-9-dependent caspase-3 activation. Caspase-3 activation in turn induced the proteolytic cleavage of its substrate such as PARP and ICAD, ladder pattern of DNA fragmentation, and an increase of SubG0/G1 phase cells. Taken together, the beneficial effect of xanthohumol might be used to combat breast cancer.

목차 (Table of Contents)

• = ABSTRACT = = 1
• I. 서론 = 3
• II. 재료 및 방법 = 5
• 1.세포주 배양 = 5
• 2.세포 생존율 측정 = 5
• 3.Flow cytometer에 의한 세포자멸사 분석 = 5
• 4.DNA 추출과 전기영동 = 6
• 5.Caspase계 cysteineprotease활성도 측정 = 6
• 6.Cytosol과 mitochondrialfraction의 분리 = 7
• 7.Westernblotting = 7
• 8.통계 처리 = 8
• III. 결과 = 9
• 1.Xanthohumol이 MDA-MB231세포 생존에 미치는 영향 = 9
• 2.Xanthohumol이 세포자멸사 미치는 영향 = 9
• 3.Xanthohumol이 caspase-3,-8,-9활성도 및 절단에 미치는 영향 = 10
• 4.Xanthohumol이 mitochonrialcytochromec와 Bax의 유리에 미치는 영향 = 10
• 5.Xanthohumol이 PARP절단 및 ICAD 발현에 미치는 영향 = 11
• 6.Caspase 억제제들이 Xanthohumol의 세포자멸사에 미치는 영향 = 12
• IV. 고찰 = 13
• V. 결론 = 18
• VI. 참고문헌 = 19