This study aimed to develop an efficient vegetative propagation protocol for Maxillaria tenuifolia. Cuttings of varying lengths — 0, 1, 2, and 3 cm — were collected from the pseudobulb base and treated with different concentrations of naphthalenea...
This study aimed to develop an efficient vegetative propagation protocol for Maxillaria tenuifolia. Cuttings of varying lengths — 0, 1, 2, and 3 cm — were collected from the pseudobulb base and treated with different concentrations of naphthaleneacetic acid (NAA): 0, 50, 100, 500, 1000, and 2000 mg·L-1. The rooting rates did not vary significantly among the cuttings of varying lengths. However, those 1-cm-long exhibited superior root development, with markedly enhanced root number, length, and diameter compared to other treatments. The shoot fresh and dry weights were also greatest in these cuttings. Rooting rates did not differ markedly among treatments with 0~–1000 mg·L -1 NAA, whereas 2000 mg·L -1 suppressed rooting rate to 78%. NAA at 500 mg·L -1 produced the highest root number, diameter, and dry weight. However, 2000 mg·L -1 inhibited shoot and root growth, including a reduction in root number, pseudobulb diameter, and biomass accumulation. These results suggest that 1-cm-long cuttings treated with 500 mg·L -1 NAA are optimal for rooting and subsequent growth for the propagation of M. tenuifolia.